Physical and genetic chromosomal maps of Streptococcus agalactiae, serotypes II and III;: rRNA operon organization

被引：7

作者：

Dmitriev, A ^{[1
]}

Suvorov, A ^{[1
]}

Totolian, A ^{[1
]}

机构：

[1] Russian Acad Med Sci, Inst Expt Med, St Petersburg 197376, Russia

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 167卷 / 01期

关键词：

group B streptococcus; pulsed field gel electrophoresis; ribosomal operon; genome mapping;

D O I：

10.1111/j.1574-6968.1998.tb13204.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A detailed analysis of two Streptococcus agalactiae (group B streptococcus, GBS) strains was performed by pulsed field gel electrophoresis (PFGE). Digestion of the chromosomal DNA with SmaI and SgrAI endonucleases, followed by separation and analysis of fragments by PFGE was carried out. Physical chromosomal maps of serotype II/(alpha+beta) and III/alpha strains of S. agalactiae were constructed. The GBS genome size was estimated to be 2200 kb. Sixteen GBS genes were used as probes and were located on the restriction maps of both strains by DNA-DNA hybridization. Six copies of ribosomal operons were found in the genome of the analyzed strains. Significant differences in the restriction patterns of chromosomal DNA and DNA-DNA hybridization between the two analyzed strains were detected so that DNA restriction patterns may be used to trace outbreaks of disease. The overall GBS chromosomal organization as determined is fairly conserved. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：33 / 39

页数：7

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