Synergism between platelets and neutrophils in provoking cardiac dysfunction after ischemia and reperfusion - Role of selectins

Background-Neutrophils (PMNs) are known to contribute to both cardiac dysfunction and myocardial necrosis after reperfusion of an ischemic heart. Moreover, platelets are:also important blood cells that can aggravate myocardial ischemic injury. This study was designed to test the effects of PMNs and platelets separately and together in provoking cardiac dysfunction in isolated perfused rat hearts after ischemia and reperfusion. Methods and Results-Control rat-hearts not subjected to ischemia were perfused without blood cells for 80 minutes. Additional control rat hearts were perfused with 75x10(6) PMNs, with 100x10(6) platelets, or with 75x10(6) PMNs + 100x10(6) platelets over a 5-minute perfusion followed by a 75-minute observation period. No significant reduction in coronary flow, left ventricular developed pressure (LVDP), or the first derivative of LVDP (dP/dt(max)) was observed at the end of the observation period in any nonischemic group. Similarly, global ischemia (I) for 20 minutes followed by 45 minutes of reperfusion (R) produced no sustained effects on the final recovery of any of these parameters in any group of hearts perfused in the absence of blood cells. However, I/R hearts perfused with either PMNs or platelets alone exhibited decreases in these variables of 10% to 12% (P<0.05 from control). Furthermore, I/R hearts perfused with both PMNs and platelets exhibited decreases of 50% to 60% in all measurements of cardiac function (P<0.001). These dual-cell-perfused VR hearts also exhibited marked increases in cardiac myeloperoxidase (MPO) activity, indicating a-significant PMN infiltration, and! enhanced P-selectin expression on the coronary microvascular endothelium. All cardiodynamic effects as well as MPO accumulation and PMN infiltration were markedly attenuated by a sialyl Lewis(x)-oligosaccharide or a recombinant soluble P-selectin ligand, which inhibits selectin-mediated cell adhesion. Conclusions-These results provide evidence that platelets and neutrophils act synergistically in provoking postreperfusion cardiac dysfunction and that this maybe largely-due to cell-to-cell interactions mediated by P-selectin. These findings may help explain the reperfusion injury phenomenon.