Quantification of the Sixth DNA Base Hydroxymethylcytosine in the Brain

被引：306

作者：

Muenzel, Martin ^{[1
]}

Globisch, Daniel ^{[1
]}

Brueckl, Tobias ^{[1
]}

Wagner, Mirko ^{[1
]}

Welzmiller, Veronika ^{[1
]}

Michalakis, Stylianos ^{[2
]}

Mueller, Markus ^{[1
]}

Biel, Martin ^{[2
]}

Carell, Thomas ^{[1
]}

机构：

[1] Univ Munich, Ctr Integrated Prot Sci CiPSM, Dept Chem, D-81377 Munich, Germany

[2] Univ Munich, Ctr Integrated Prot Sci CiPSM, Dept Pharm, D-81377 Munich, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 31期

关键词：

demethylation; DNA methylation; DNA modification; epigenetics; hydroxymethylcytosine; METHYLATION; 5-HYDROXYMETHYLCYTOSINE; 5-METHYLCYTOSINE; EPIGENETICS; OXIDATION; ANALOGS;

D O I：

10.1002/anie.201002033

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Mind over matter: LC-MS has allowed the amount of the post-replicatively formed DNA base 5hydroxymethylcytosine (see picture; left) to be quantified in brain tissue. The nucleoside is most abundant in areas that are associated with higher cognitive functions, and its content in mouse hippocampi seems to increase with age. The new method enables hydroxymethylcytosine to be quantified with unprecedented accuracy. (Figure Presented) © 2010 Wiley-vCH Verlag GmbH & Co. KGaA.

引用

页码：5375 / 5377

页数：3

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