The kinetics of DNA-cationic vesicle complex formation

被引:90
作者
Barreleiro, PCA [1 ]
Lindman, B [1 ]
机构
[1] Lund Univ, Ctr Chem & Chem Engn, S-22100 Lund, Sweden
关键词
D O I
10.1021/jp0277107
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Stopped-flow fluorescence studies were performed to investigate the kinetics of formation of complexes between DNA and cationic vesicles. We followed the kinetics as a function of charge ratio and lipid vesicle composition. Binary mixtures of a saturated cationic lipid, dioctadecyldimethlammonium bromide (DODAB), and a zwitterionic lipid, dioleoylphosphatidylethanolamine (DOPE), were used to prepare small unilamellar vesicles at 25 degreesC. The binding followed first-order reactions and occurred stepwise. The results presented indicate the existence of a three-step mechanism for cationic vesicle "binding" to DNA: first DNA rapidly adsorbs onto the oppositely charged membrane surface (milliseconds), and thereafter, on a longer time scale (seconds), the formed DNA-lipid complex continues to aggregate and grow; a third step is also observed, which seems to be due to rearrangement of the complex with a further change of the DNA conformation. The time constant of each step was determined by the relative amounts of DNA and cationic vesicles and the composition of the lipid bilayer of the vesicles.
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收藏
页码:6208 / 6213
页数:6
相关论文
共 36 条
[1]   Effects of supercoiling in electrophoretic trapping of circular DNA in polyacrylamide gels [J].
Åkerman, B .
BIOPHYSICAL JOURNAL, 1998, 74 (06) :3140-3151
[2]   DNA-induced endocytosis upon local microinjection to giant unilamellar cationic vesicles [J].
Angelova, MI ;
Hristova, N ;
Tsoneva, I .
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1999, 28 (02) :142-150
[3]   Interaction of DNA with cationic vesicles: A calorimetric study [J].
Barreleiro, PCA ;
Olofsson, G ;
Alexandridis, P .
JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (32) :7795-7802
[4]   Mechanism of formation of DNA-cationic vesicle complexes [J].
Barreleiro, PCA ;
May, RP ;
Lindman, B .
FARADAY DISCUSSIONS, 2003, 122 :191-201
[5]  
BARRELEIRO PCA, 2000, ILL ANN REP, P54
[6]   Interaction of surfactants with DNA. Role of hydrophobicity and surface charge on intercalation and DNA melting [J].
Bhattacharya, S ;
Mandal, SS .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1323 (01) :29-44
[7]   CONDENSATION OF DNA BY MULTIVALENT CATIONS - CONSIDERATIONS ON MECHANISM [J].
BLOOMFIELD, VA .
BIOPOLYMERS, 1991, 31 (13) :1471-1481
[8]   Stopped-flow fluorometric study of the interaction of melittin with phospholipid bilayers: Importance of the physical state of the bilayer and the acyl chain length [J].
Bradrick, TD ;
Philippetis, A ;
Georghiou, S .
BIOPHYSICAL JOURNAL, 1995, 69 (05) :1999-2010
[9]   Molecular assembling of DNA with amphipathic peptides [J].
Dufourcq, J ;
Neri, W ;
Henry-Toulmé, N .
FEBS LETTERS, 1998, 421 (01) :7-11
[10]   Biophysical characterization of cationic lipid:DNA complexes [J].
Eastman, SJ ;
Siegel, C ;
Tousignant, J ;
Smith, AE ;
Cheng, SH ;
Scheule, RK .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1325 (01) :41-62