Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation

被引：31

作者：

Dobano, Carlota ^{[1
]}

Widera, Georg

Rabussa, Dietmar

Doolan, Denise L.

机构：

[1] Univ Barcelona, Hosp Clin IDIBAPS, Ctr recerca Salut Int Barcelona CRESIB, E-08036 Barcelona, Spain

[2] Naval Med Res Ctr, Malaria Program, Silver Spring, MD 20910 USA

[3] Henry M Jackson Fdn, Rockville, MD 20852 USA

[4] Genetronics Inc, San Diego, CA 92121 USA

[5] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21205 USA

来源：

VACCINE | 2007年 / 25卷 / 36期

关键词：

malaria; DNA vaccine; electroporation;

D O I：

10.1016/j.vaccine.2007.06.036

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP 17, respectively. Immunization with 5 mu g of DNA via EP was equivalent to 50 mu g of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-gamma responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents. (c) 2007 Elsevier Ltd. All rights reserved.

引用

收藏

页码：6635 / 6645

页数：11

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