Quantitative HPLC analysis of acids in Brazilian cachacas and various spirits using fluorescence detection of their 9-anthrylmethyl esters

被引：19

作者：

do Nascimento, RJ

Cardoso, DR

De Keukeleire, D

Lima-Neto, BD

Franco, DW

机构：

[1] Univ Sao Paulo, Dept Quim & Fis Mol, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil

[2] Univ Ghent, Fac Pharmaceut Sci, B-9000 Ghent, Belgium

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 12期

关键词：

HPLC; acids; fluorescence; 9-anthrylmethyl esters; spirits;

D O I：

10.1021/jf9905267

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

An analytical procedure for the separation and quantification of 17 short-chain, medium-chain, and long-chain acids in cachacas and various spirits has been developed involving C18 solid phase extraction, derivatization with 9-anthryldiazomethane, and reverse phase HPLC using fluorescence detection. The limit of detection was between 5 and 15 fmol, whereas the recovery of nonanoic acid as internal standard was > 95%. Relative standard deviation values for reproducibility were between 0.09 and 20.4%, and repeatability was between 0.05 and 11.3%.

引用

页码：6070 / 6073

页数：4

共 27 条

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Antonelli A, 1995, CHROMATOGRAPHIA, V41, P722, DOI 10.1007/BF02267811

[2] 9-DIAZOMETHYLANTHRACENE AS A NEW FLUORESCENCE AND ULTRAVIOLET LABEL FOR THE SPECTROMETRIC DETECTION OF PICOMOLE QUANTITIES OF FATTY-ACIDS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].