Facile disulfide bond cleavage in gaseous peptide and protein cations by ultraviolet photodissociation at 157 nm

被引：94

作者：

Fung, YME

Kjeldsen, F ^{[1
]}

Silivra, OA

Chan, TWD

Zubarev, RA

机构：

[1] Univ Uppsala, Lab Biol & Med Mass Spectrometry, BMC, S-75123 Uppsala, Sweden

[2] Chinese Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 39期

关键词：

mass spectrometry; photodissociation; proteins; radical chemistry; sequencing;

D O I：

10.1002/anie.200501533

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Overall charge unchanged: Disulfide bonds in gaseous polypeptide cations can be cleaved efficiently by ultraviolet photodissociation (UVPD) at 157 nm (see scheme). Unlike electron-capture dissociation, UVPD cleaves S-S bonds without overall charge reduction in the product ions. This cleavage is suggested to be a result of electronic excitation caused by the absorption of photons of 7.9 eV. (Figure Presented) © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：6399 / 6403

页数：5

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