Enhanced tolerance to drought stress in transgenic tobacco plants overexpressing VTE1 for increased tocopherol production from Arabidopsis thaliana

被引：92

作者：

Liu, Xiaoli ^{[1
,2
]}

Hua, Xuejun ^{[1
]}

Guo, Juan ^{[1
,2
]}

Qi, Dongmei ^{[1
]}

Wang, Lijuan ^{[1
,2
]}

Liu, Zhipeng ^{[1
,2
]}

Jin, Zhiping ^{[1
]}

Chen, Shuangyan ^{[1
]}

Liu, Gongshe ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Bot, Key Lab Photosynthesis & Environm Mol Physiol, Beijing 100093, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100093, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 07期

基金：

中国国家自然科学基金;

关键词：

drought; stress tolerance; tobacco; tocopherols; tocopherol cyclase gene;

D O I：

10.1007/s10529-008-9672-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tocopherol cyclase (VTE1, encoded by VTE1 gene) catalyzes the penultimate step of tocopherol synthesis. Transgenic tobacco plants overexpressing VTE1 from Arabidopsis were exposed to drought conditions during which transgenic lines had decreased lipid peroxidation, electrolyte leakage and H2O2 content, but had increased chlorophyll compared with the wild type. Thus VTE1 can be used to increase vitamin E content of plants and also to enhance tolerance to environmental stresses.

引用

收藏

页码：1275 / 1280

页数：6

相关论文

共 16 条

[1] Specific roles of α- and γ-tocopherol in abiotic stress responses of transgenic tobacco [J].

Abbasi, Ali-Reza ;

Hajirezaei, Mohamad ;

Hofius, Daniel ;

Sonnewald, Uwe ;

Voll, Lars M. .

PLANT PHYSIOLOGY, 2007, 143 (04) :1720-1738

[2] Characterization of an Arabidopsis mutant deficient in γ-tocopherol methyltransferase [J].

Bergmüller, E ;

Porfirova, S ;

Dörmann, P .

PLANT MOLECULAR BIOLOGY, 2003, 52 (06) :1181-1190

[3] Progress of vitamin E metabolic engineering in plants [J].

Chen, Shuangyan ;

Li, Hongjie ;

Liu, Gongshe .

TRANSGENIC RESEARCH, 2006, 15 (06) :655-665

[4] Vitamin E is essential for the tolerance of Arabidopsis thaliana to metal-induced oxidative stress [J].

Collin, Valerie C. ;

Eymery, Francoise ;

Genty, Bernard ;

Rey, Pascal ;

Havaux, Michel .

PLANT CELL AND ENVIRONMENT, 2008, 31 (02) :244-257

[5] Vitamin synthesis in plants: Tocopherols and carotenoids [J].

DellaPenna, Dean ;

Pogson, Barry J. .

ANNUAL REVIEW OF PLANT BIOLOGY, 2006, 57 :711-738

[6]

GUO J, 2006, J APPL ENV BIOL, V12, P468

[7] Vitamin E protects against photoinhibition and photooxidative stress in Arabidopsis thaliana [J].

Havaux, M ;

Eymery, F ;

Porfirova, S ;

Rey, P ;

Dörmann, P .

PLANT CELL, 2005, 17 (12) :3451-3469

[8] Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds [J].

Hodges, DM ;

DeLong, JM ;

Forney, CF ;

Prange, RK .

PLANTA, 1999, 207 (04) :604-611

[9] Alterations in tocopherol cyclase activity in transgenic and mutant plants of arabidopsis affect tocopherol content, tocopherol composition, and oxidative stress [J].

Kanwischer, M ;

Porfirova, S ;

Bergmüller, E ;

Dörmann, P .

PLANT PHYSIOLOGY, 2005, 137 (02) :713-723

[10] Genetic manipulation of proline levels affects antioxidants in soybean subjected to simultaneous drought and heat stresses [J].

Kocsy, G ;

Laurie, R ;

Szalai, G ;

Szilágyi, V ;

Simon-Sarkadi, L ;

Galiba, G ;

de Ronde, JA .

PHYSIOLOGIA PLANTARUM, 2005, 124 (02) :227-235