Cloning of a human tRNA isopentenyl transferase

被引：60

作者：

Golovko, A ^{[1
]}

Hjälm, G ^{[1
]}

Sitbon, F ^{[1
]}

Nicander, B ^{[1
]}

机构：

[1] SLU, Dept Plant Biol, SE-75007 Uppsala, Sweden

来源：

GENE | 2000年 / 258卷 / 1-2期

关键词：

complementation; cytokinin; isopentenyladenosine; suppressor tRNA; tRNA modification;

D O I：

10.1016/S0378-1119(00)00421-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cDNA of human origin is shown to encode a tRNA isopentenyl transferase (E.C. 2.5.1.8). Expression of the gene in a Saccharomyces cerevisiae mutant lacking the endogenous tRNA isopentenyl transferase MOD5 resulted in functional complementation and reintroduction of isopentenyladenosine into tRNA. The deduced amino acid sequence contains a number of regions conserved in known tRNA isopentenyl transferases. The similarity to the S. cerevisiae MOD5 protein is 53%, and to the Escherichia coli MiaA protein 47%. The human sequence was found to contain a single C2H2 Zn-finger-like motif, which was detected also in the MOD5 protein, and several putative tRNA transferases located by BLAST searches, but not in prokaryotic homologues. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：85 / 93

页数：9

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