Hybridization dependent cleavage of internally modified disulfide-peptide nucleic acids

被引：4

作者：

Boll, I ^{[1
]}

Krämer, R ^{[1
]}

Mokhir, A ^{[1
]}

机构：

[1] Heidelberg Univ, Chirurg Klin, D-69120 Heidelberg, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2005年 / 15卷 / 03期

关键词：

peptide nucleic acid; disulfide cleavage; DNA analysis;

D O I：

10.1016/j.bmcl.2004.11.066

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Selectivity of the cleavage of single stranded over hybridized forms of internally modified disulfide-peptide nucleic acids (PNA) has been optimized using a series of phosphines and thiols, which have different sizes and charges. For the most selective cleaver found (tris-(carboxyethyl)-phosphine), reactivity of single stranded PNA is 33 times higher than that of the PNA-DNA duplex. Selectivity of single stranded disulfide-PNA cleavage has been explained in terms of electrostatic interaction between the substrate and the cleaver. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：505 / 509

页数：5

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