Rapid preparation of giant unilamellar vesicles

被引：268

作者：

Moscho, A ^{[1
]}

Orwar, O ^{[1
]}

Chiu, DT ^{[1
]}

Modi, BP ^{[1
]}

Zare, RN ^{[1
]}

机构：

[1] STANFORD UNIV,DEPT CHEM,STANFORD,CA 94305

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 21期

关键词：

liposomes; encapsulation; proteoliposomes; transport;

D O I：

10.1073/pnas.93.21.11443

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report here a rapid evaporation method that produces in high yield giant unilamellar vesicles up to 50 mu m in diameter. The vesicles are obtained after only 2 min and can be prepared from different phospholipids, including L-alpha-phosphatidylcholine (lecithin), dipalmitoleoyl L-alpha-phosphatidylcholine, and beta-arachidonoyl gamma-palmitoyl L-alpha-phosphatidylcholine. Vesicles can be produced in distilled water and in Hepes, phosphate, and berate buffers in the pH range of 7.0 to 11.5 with ionic strengths up to 50 mM. The short preparation time allows encapsulation of labile molecular targets or enzymes with high catalytic activities. Cell-sized proteoliposomes have been prepared in which gamma-glutamyltransferase (EC 2.3.2.2) was functionally incorporated into the membrane wall.

引用

页码：11443 / 11447

页数：5

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[2]

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