Enhanced antitumor effects by the coculture of allotumor RNA-pulsed dendritic cells with autologous cytokine-induced killer cells on hormone-refractory prostate cancer

被引：10

作者：

Pang, Jun ^{[1
]}

Gao, Xin ^{[1
]}

Liu, Xiaopeng ^{[1
]}

Wang, Kebing ^{[1
]}

Zhang, Yan ^{[1
]}

Feng, Lianqiang ^{[2
]}

Zhan, Hailun ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Urol, Guangzhou 51063, Peoples R China

[2] Sun Yat Sen Univ, Basic Med Inst, Dept Immunol, Guangzhou, Peoples R China

来源：

CANCER INVESTIGATION | 2007年 / 25卷 / 07期

基金：

中国国家自然科学基金;

关键词：

dendritic cells; cytokine-induced killer cells; hormone-refractory prostate cancer; immunotherapy;

D O I：

10.1080/07357900701511789

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

In this study, we evaluated antitumor effects of allotumour RNA-transfected dendritic cells (DCs) cocultured with autologous cytokine-induced killer cells (CIKs) on hormone-refractory prostate cancer. The cocultured cells enhanced prostate cancer cytolysis from 26% (CIKs-induced cytolysis) to 80.8%. They also increased the productions of CD4(+) Th1 (IFN-gamma+IL-4(-), 55.52%) and CD8(+) T (IFN-gamma(+), 69.59%) cells determined by intracellular cytokines IFN-gamma/IL-4 staining and reduced the rate of CD4(+)CD25(+) cells from 18.72% (in CIKs) to 9.72%. The cocultured cells significantly inhibited tumor growth in SCID mouse and induced cancer cells necrosis and apoptosis. Our study indicates that tumor RNA-pulsed DCs cocultured with autologous CIKs significantly enhance antitumor immunity, which can be induced by increased CD4(+) Th1 and CD8(+) T cells and decreased CD4(+)CD25(+) regulatory T (T-reg) cells. This provides a potential immunotherapy strategy for HRPC.

引用

页码：527 / 534

页数：8

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