Effects of estrogen on leptin gene promoter activation in MCF-7 breast cancer and JEG-3 choriocarcinoma cells:: selective regulation via estrogen receptors α and β

被引:50
作者
O'Neil, JS
Burow, ME
Green, AE
McLachlan, JA
Henson, MC
机构
[1] Tulane Univ, Ctr Hlth Sci, Dept Obstet & Gynecol SC 11, New Orleans, LA 70112 USA
[2] Tulane Univ, Hlth Sci Ctr, Dept Physiol, New Orleans, LA 70112 USA
[3] Tulane Univ, Hlth Sci Ctr, Dept Struct & Cellular Biol, New Orleans, LA 70112 USA
[4] Tulane Univ, Hlth Sci Ctr, Dept Pharmacol, New Orleans, LA 70112 USA
[5] Tulane Univ, Hlth Sci Ctr, Interdisciplinary Program Mol & Cellular Biol, New Orleans, LA 70112 USA
[6] Tulane Univ, Hlth Sci Ctr, Tulane Xavier Ctr Bioenvironm Res, New Orleans, LA 70112 USA
关键词
leptin; estrogen; antiestrogens; MCF-7 (breast cancer cells); JEG-3 (choriocarcinoma cells);
D O I
10.1016/S0303-7207(01)00473-7
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Leptin is a potential regulator. of conceptus development. We have previously suggested that in primate pregnancy, leptin biosynthesis is regulated by estrogen in a tissue-specific manner. Therefore, the objective of the current study was to determine the mechanism of estrogen action on LEP promoter activation in divergent cell types. The effects of estrogen were investigated in estrogen receptor (ER)-positive MCF-7 breast cancer cells and in ER-negative JEG-3 choriocarcinoma cells. Cells were transfected with a leptin-luciferase or an estrogen responsive element (ERE)-luciferase reporter construct, in conjunction with ER alpha, ER beta, or empty vector expression plasmids. Cells were treated with estradiol and/or the specific estrogen antagonists, ICI-182,780 or 4-hydroytamoxifen. In MCF-7 cells, estradiol stimulated (P < 0.05) ERE-luciferase activity and was inhibited by ICI-182,780, but did not stimulate leptin-luciferase activity. However, leptin-luciferase was stimulated by estradiol (P < 0.05) and inhibited by antiestrogens in JEG-3 cells that were co-transfected with ER alpha. Both antiestrogens stimulated leptin-luciferase activity (P < 0.05) in JEG-3 cells co-transfected with ER<beta>. Results suggested that LEP promoter activation may depend upon co-activators present in leptin-producing cells and may be inhibited by repressors present in non-leptin producing cells. Divergent, effects of estrogen may be owed to differences in the type of ER (alpha or beta) expressed in target tissues. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:67 / 75
页数:9
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