Rapid characterization of artemether and its in vitro metabolites on incubation with bovine hemoglobin, rat blood and dog blood by capillary gas chromatography chemical ionization mass spectrometry

被引：15

作者：

Blum, W ^{[1
]}

Pfaar, U ^{[1
]}

Kühnöl, J ^{[1
]}

机构：

[1] Novartis Pharma AG, Dept Res, CH-4002 Basel, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1998年 / 710卷 / 1-2期

关键词：

artemether;

D O I：

10.1016/S0378-4347(98)00113-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast and sensitive analytical method was developed to characterize artemether and its metabolites in small amounts in body fluids. The extracts were derivatized with N-methyl-N-trimethylsilyltrifluoroacetamide, separated on an optimized capillary gas chromatographic system and identified by chemical ionization mass spectrometry by using ammonia as reagent gas. The analytical assay is demonstrated on samples extracted from bovine hemoglobin, rat blood and dog blood. Full mass spectra of artemether and three metabolites were obtained at a level of 1.10(-6) g/ml. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：101 / 113

页数：13

共 41 条

[1] REDUCTIVE ELECTROCHEMICAL HPLC ASSAY FOR ARTEMISININ (QINGHAOSU) [J].

ACTON, N ;

KLAYMAN, DL ;

ROLLMAN, IJ .

PLANTA MEDICA, 1985, 51 (05) :445-446

[2] ARTEMISITENE, A NEW SESQUITERPENE LACTONE ENDOPEROXIDE FROM ARTEMISIA-ANNUA [J].

ACTON, N ;

KLAYMAN, DL .

PLANTA MEDICA, 1985, 51 (05) :441-442

[3] REACTION OF ANTIMALARIAL ENDOPEROXIDES WITH SPECIFIC PARASITE PROTEINS [J].

ASAWAMAHASAKDA, W ;

ITTARAT, I ;

PU, YM ;

ZIFFER, H ;

MESHNICK, SR .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (08) :1854-1858

[4] 2 UNEXPECTED COUMARIN DERIVATIVES FROM TISSUE-CULTURES OF COMPOSITAE SPECIES [J].

BANTHORPE, DV ;

BROWN, GD .

PHYTOCHEMISTRY, 1989, 28 (11) :3003-3007

[5] PREPARATION OF INERT AND HIGH-TEMPERATURE STABLE APOLAR AND MEDIUM POLAR GLASS-CAPILLARY COLUMNS USING OH-TERMINATED POLYSILOXANE STATIONARY PHASES [J].

BLUM, W .

JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1985, 8 (11) :718-726

[6]

BLUM W, 1991, HOCHTEMPERATUR GAS C, P79

[7]

BLUM W, 1990, J HIGH RES CHROMATOG, V13, P85

[8] GERMPLASM VARIATION IN ARTEMISININ CONTENT OF ARTEMISIA-ANNUA USING AN ALTERNATIVE METHOD OF ARTEMISININ ANALYSIS FROM CRUDE PLANT-EXTRACTS [J].

CHARLES, DJ ;

SIMON, JE ;

WOOD, KV ;

HEINSTEIN, P .

JOURNAL OF NATURAL PRODUCTS, 1990, 53 (01) :157-160

[9] IDENTIFICATION OF THE INVIVO METABOLITES OF THE ANTIMALARIAL ARTEETHER BY THERMOSPRAY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY [J].

CHI, HT ;

RAMU, K ;

BAKER, JK ;

HUFFORD, CD ;

LEE, IS ;

ZENG, YL ;

MCCHESNEY, JD .

BIOLOGICAL MASS SPECTROMETRY, 1991, 20 (10) :609-628

[10] Clinical pharmacology and therapeutic potential of artemisinin and its derivatives in the treatment of malaria [J].

deVries, PJ ;

Dien, TK .

DRUGS, 1996, 52 (06) :818-836

← 1 2 3 4 5 →