Serum-free recombinant production of adenovirus using a hollow fiber capillary system

被引：12

作者：

Gardner, TA

Ko, SC

Yang, L

Cadwell, JJS

Chung, LWK

Kao, C

机构：

[1] Indiana Univ, Med Ctr, Dept Urol, Indianapolis, IN 46202 USA

[2] Univ Virginia, Hlth Sci Ctr, Charlottesville, VA USA

[3] FiberCell Syst, New Market, MD USA

来源：

BIOTECHNIQUES | 2001年 / 30卷 / 02期

关键词：

D O I：

10.2144/01302pf01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A novel rnethod for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory nse and has the potential to scale-up the adenovirus production for clinical use.

引用

页码：422 / +

页数：5

共 7 条

[1]

Becker, 1994, J Thromb Thrombolysis, V1, P7

[2]

GARDNER TA, 1998, GENE THER MOL BIOL, V2, P41

[3]

SCALE-UP OF THE ADENOVIRUS EXPRESSION SYSTEM FOR THE PRODUCTION OF RECOMBINANT PROTEIN IN HUMAN 293S CELLS [J].