Long distance selective fiber outgrowth of transplanted hNT neurons in white matter tracts of the adult rat brain

Terminally differentiated neurons derived from a human teratocarcinoma cell line (NT2N or hNT neurons) are promising as a cell source for transplantation, as they have been shown to be safe for transplantation in humans. We have shown previously that hNT neurons can express a catecholaminergic phenotype in a rat Parkinson model. In this study, we investigated the long-term, survival and ability of hNT neurons to. express tyrosine hydroxylase and reconstruct the, dopamine-denervated nigrostriatal pathway. Hemiparkinsonian rats received grafts of 400,000,viable hNT neurons,into each of the denervated striatum and substantia. nigra. Robust hNT grafts were detected up to 24 weeks posttransplantation, although few cells expressed tyrosine hydroxylase; Many hNT fibers were often associated with ipsilateral and contralateral white matter tracts-corpus callosum, rostral migratory stream, optic tract,. and external capsule. Fewer fibers were associated with the superior cerebellar peduncle, medial lemniscus, and nigrostriatal, pathway. Axons also projected into the frontal cortex and extended parallel to the, surface of the brain in the superficial cortical layers These pathways were seen in all, grafted animals, suggesting that specific guidance cues exist in the adult brain governing hNT fiber outgrowth. Injured adult axons and transplanted embryonic neuronal axons rarely extend for such distances in the adult nervous systems We propose that elucidating the factors promoting and guiding hNT axonal outgrowth, could provide important clues to enhancing regeneration and target reinnervation in the adult brain, two factors of critical importance for cell restoration strategies aimed at brain repair. (c) 2005 Wiley-Liss, Inc.