N-acetylcysteine inhibits angiotensin II-mediated activation of extracellular signal-regulated kinase and epidermal growth factor receptor

被引：45

作者：

Frank, GD

Eguchi, S

Inagami, T

Motley, ED ^{[1
]}

机构：

[1] Meharry Med Coll, Dept Anat & Physiol, Nashville, TN 37208 USA

[2] Vanderbilt Univ, Sch Med, Dept Biochem, Nashville, TN 37232 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 280卷 / 04期

关键词：

angiotensin II; EGF receptor; extracellular signal-regulated kinase; N-acetylcysteine; reactive oxygen species; vascular smooth muscle cells;

D O I：

10.1006/bbrc.2001.4251

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Angiotensin II (Ang II) is known to stimulate reactive oxygen species (ROS) generation and epidermal growth factor (EGF) receptor transactivation to mediate growth-promoting signals such as extracellular signal-regulated kinase (ERK) in vascular smooth muscle cells (VSMCs). However, how ROS and EGF receptor interact to orchestrate these signals in VSMCs remains unclear. Mere we found that an antioxidant, N-acetylcysteine, inhibited ERK activation and EGF receptor tyrosine phosphorylation induced by Ang II. Moreover, H2O2 stimulates EGF receptor tyrosine phosphorylation and EGF receptor inhibitors attenuated H2O2-induced ERK activation. These data indicate that ROS mediate Ang II-induced EGF receptor transactivation, a critical mechanism for ERK-dependent growth in VSMCs. (C) zool Academic Press.

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页码：1116 / 1119

页数：4

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