Ly-49W, an activating receptor of nonobese diabetic mice with close homology to the inhibitory receptor Ly-49G, recognizes H-2Dk and H-2Dd

被引：34

作者：

Silver, ET ^{[1
]}

Gong, DE ^{[1
]}

Hazes, B ^{[1
]}

Kane, KP ^{[1
]}

机构：

[1] Univ Alberta, Dept Med Microbiol & Immunol, Edmonton, AB T6G 2S2, Canada

来源：

JOURNAL OF IMMUNOLOGY | 2001年 / 166卷 / 04期

关键词：

D O I：

10.4049/jimmunol.166.4.2333

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The diversity and ligand specificity of activating Ly-49 receptors expressed by murine NK cells are largely unknown. We cloned a new Ly-49-activating receptor, expressed by NK cells of the nonobese diabetic mouse strain, which we have designated Ly-49W, Ly-49W is highly related to the known inhibitory receptor Ly-49G in its carbohydrate recognition domain, exhibiting 97.6% amino acid identity in this region. We demonstrate that the 4D11 and Cwy-3 Abs, thought to he Ly-49G specific, also recognize Ly-49W, Rat RNK-16 cells transfected with Ly-49W mediated reverse Ab-dependent cellular cytotoxicity of FcR-positive target cells, indicating that Ly-49W can activate NK-mediated lysis. We further show that Ly-49W is allo-MHC specific: Ly-49W transfectants of RNK-16 only lysed Con A blasts expressing H-2(k) or H-2(d) haplotypes, and Ab-blocking experiments indicated that H-2D(k) and D-d are ligands for Ly-49W, Ly-49W is the first activating Ly-49 receptor demonstrated to recognize an H-2k class I product. Ly-49G and Ly-49W represent a new pair of NK receptors with very similar ligand-binding domains, but opposite signaling functions.

引用

页码：2333 / 2341

页数：9

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