Chondrocyte expressed protein-68 (CEP-68), a novel human marker gene for cultured chondrocytes

被引：39

作者：

Steck, E

Benz, K

Lorenz, H

Loew, M

Gress, T

Richter, W

机构：

[1] Heidelberg Univ, Dept Orthopaed Surg, D-69118 Heidelberg, Germany

[2] Univ Ulm, Dept Internal Med 1, D-89081 Ulm, Germany

来源：

BIOCHEMICAL JOURNAL | 2001年 / 353卷

关键词：

cartilage; chondrogenic phenotype; chondro-induction; mesenchymal stem cells; representational difference analysis;

D O I：

10.1042/0264-6021:3530169

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the search for new marker genes suitable to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture, we have identified and characterized a novel gene called chondrocyte expressed protein-68 (CEP-68), harbouring an N-terminal leader peptide and an epidermal growth factor-like calcium-binding domain. CEP-68 defines a new family of proteins and complements collagen type II as a new marker for stem-cell-based chondrogenic tissue engineering.

引用

收藏

页码：169 / 174

页数：6

相关论文

共 21 条

[1] FIBULIN, A NOVEL PROTEIN THAT INTERACTS WITH THE FIBRONECTIN RECEPTOR-BETA SUBUNIT CYTOPLASMIC DOMAIN [J].

ARGRAVES, WS ;

DICKERSON, K ;

BURGESS, WH ;

RUOSLAHTI, E .

CELL, 1989, 58 (04) :623-629

[2] DEDIFFERENTIATED CHONDROCYTES REEXPRESS THE DIFFERENTIATED COLLAGEN PHENOTYPE WHEN CULTURED IN AGAROSE GELS [J].

BENYA, PD ;

SHAFFER, JD .

CELL, 1982, 30 (01) :215-224

[3]

BENZ K, 2000, OSTEOLOGIE, V9, P97

[4] PRODUCTION OF OSTEOCALCIN BY HUMAN-BONE CELLS-INVITRO - EFFECTS OF 1,25(OH)2D3, 24,25(OH)2D3, PARATHYROID-HORMONE, AND GLUCOCORTICOIDS [J].

BERESFORD, JN ;

GALLAGHER, JA ;

POSER, JW ;

RUSSELL, RGG .

METABOLIC BONE DISEASE & RELATED RESEARCH, 1984, 5 (05) :229-234

[5] TREATMENT OF DEEP CARTILAGE DEFECTS IN THE KNEE WITH AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].

BRITTBERG, M ;

LINDAHL, A ;

NILSSON, A ;

OHLSSON, C ;

ISAKSSON, O ;

PETERSON, L .

NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (14) :889-895

[6] cDNA-RDA of genes expressed in fetal and adult lungs identifies factors important in development and function [J].

Cooper, P ;

Mueck, B ;

Yousefi, S ;

Potter, S ;

Jarai, G .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2000, 278 (02) :L284-L293

[7] Identification of human testis-specific transcripts and analysis of their expression in tumor cells [J].

De Smet, C ;

Martelange, V ;

Lucas, S ;

Brasseur, F ;

Lurquin, C ;

Boon, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 241 (03) :653-657

[8] Isolation of differentially expressed genes by combining representational difference analysis (RDA) and cDNA library arrays [J].

Geng, M ;

Wallrapp, C ;

Müller-Pillasch, F ;

Frohme, M ;

Hoheisel, JD ;

Gress, TM .

BIOTECHNIQUES, 1998, 25 (03) :434-438

[9] THE REPAIR OF EXPERIMENTALLY PRODUCED DEFECTS IN RABBIT ARTICULAR-CARTILAGE BY AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].

GRANDE, DA ;

PITMAN, MI ;

PETERSON, L ;

MENCHE, D ;

KLEIN, M .

JOURNAL OF ORTHOPAEDIC RESEARCH, 1989, 7 (02) :208-218

[10]

HAKALA BE, 1993, J BIOL CHEM, V268, P25803