In vitro hydrogen production by glucose dehydrogenase and hydrogenase

被引：64

作者：

Woodward, J

Mattingly, SM

Danson, M

Hough, D

Ward, N

Adams, M

机构：

[1] UNIV BATH,DEPT BIOCHEM,BATH BA2 7AY,AVON,ENGLAND

[2] UNIV GEORGIA,DEPT BIOCHEM,ATHENS,GA 30602

[3] UNIV GEORGIA,CTR METALLOENZYME STUDIES,ATHENS,GA 30602

来源：

NATURE BIOTECHNOLOGY | 1996年 / 14卷 / 07期

关键词：

glucose dehydrogenase; hydrogenase; hydrogen production; gluconic acid;

D O I：

10.1038/nbt0796-872

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity.

引用

页码：872 / 874

页数：3

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