Essential carboxyl and tryptophan residues in Jack Bean α-D-mannosidase

被引：7

作者：

Burrows, HM ^{[1
]}

Rastall, RA ^{[1
]}

机构：

[1] Univ Reading, Dept Food Sci & Technol, Reading RG6 6AP, Berks, England

来源：

PHYTOCHEMISTRY | 1998年 / 48卷 / 02期

关键词：

Canavalia ensiformis; Leguminosae; Jack Bean; modification; glycosidase; alpha-mannosidase;

D O I：

10.1016/S0031-9422(97)01095-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Mannosidase from Canavalia ensiformis was chemically modified using carboxyl group-specific carbodiimides and tryptophan specific N-bromosuccinimide. Data consistent with the presence of one essential carboxyl residue and 4 tryptophan residues was obtained. N-terminal sequencing studies indicated that the enzyme exists as a dimer with subunit M-r of 110,000. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：257 / 260

页数：4

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