Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal cord tissue

被引:33
作者
Bogen, O
Dreger, M
Gillen, C
Schröder, W
Hucho, F
机构
[1] Free Univ Berlin, Inst Chem Biochem, D-14195 Berlin, Germany
[2] Res & Dev Grunenthal GmbH, Aachen, Germany
关键词
IB4; versican; nonpeptidergic C-fibers; extracellular matrix; neuropathic pain;
D O I
10.1111/j.1742-4658.2005.04543.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nociceptors are specialized nerve fibers that transmit noxious pain stimuli to the dorsal horn of the spinal cord. A subset of nociceptors, the nonpeptidergic C-fibers, is characterized by its reactivity for the plant isolectin B4 (IB4) from Griffonia simplicifolia. The molecular nature of the IB4-reactive glycoconjugate, although used as a neuroanatomical marker for more than a decade, has remained unknown. We here present data which strongly suggest that a splice variant of the extracellular matrix proteoglycan versican is the IB4-reactive glycoconjugate associated with these nociceptors. We isolated (by subcellular fractionation and IB4 affinity chromatography) a glycoconjugate from porcine spinal cord tissue that migrated in SDS/PAGE as a single distinct protein band at an apparent molecular mass of > 250 kDa. By using MALDI-TOF/TOF MS, we identified this glycoconjugate unambiguously as a V2-like variant of versican. Moreover, we demonstrate that the IB4-reactive glycoconjugate and the versican variant can be co-released from spinal cord membranes by hyaluronidase, and that the IB4-reactive glycoconjugate and the versican variant can be co-precipitated by an anti-versican immunoglobulin and perfectly co-migrate in SDS/PAGE. Our findings shed new light on the role of the extracellular matrix, which is thought to be involved in plastic changes underlying pain-related phenomena such as hyperalgesia and allodynia.
引用
收藏
页码:1090 / 1102
页数:13
相关论文
共 44 条
[1]  
Amir R, 2000, NEUROSCIENCE, V95, P189
[2]   EXTRACELLULAR-MATRIX OF CENTRAL-NERVOUS-SYSTEM WHITE MATTER - DEMONSTRATION OF AN HYALURONATE-PROTEIN COMPLEX [J].
ASHER, R ;
PERIDES, G ;
VANDERHAEGHEN, JJ ;
BIGNAMI, A .
JOURNAL OF NEUROSCIENCE RESEARCH, 1991, 28 (03) :410-421
[3]  
Bennett DLH, 1998, J NEUROSCI, V18, P3059
[4]   A PERIPHERAL MONONEUROPATHY IN RAT THAT PRODUCES DISORDERS OF PAIN SENSATION LIKE THOSE SEEN IN MAN [J].
BENNETT, GJ ;
XIE, YK .
PAIN, 1988, 33 (01) :87-107
[5]   Potent analgesic effects of GDNF in neuropathic pain states [J].
Boucher, TJ ;
Okuse, K ;
Bennett, DLH ;
Munson, JB ;
Wood, JN ;
McMahon, SB .
SCIENCE, 2000, 290 (5489) :124-127
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   Perineuronal nets: past and present [J].
Celio, MR ;
Spreafico, R ;
De Biasi, S ;
Vitellaro-Zuccarello, L .
TRENDS IN NEUROSCIENCES, 1998, 21 (12) :510-514
[8]   Integrin signaling in inflammatory and neuropathic pain in the rat [J].
Dina, OA ;
Parada, CA ;
Yeh, J ;
Chen, XJ ;
McCarter, GC ;
Levine, JD .
EUROPEAN JOURNAL OF NEUROSCIENCE, 2004, 19 (03) :634-642
[9]  
DOURSZIMMERMANN MT, 1994, J BIOL CHEM, V269, P32992
[10]   Identification of some lectin IB4 binding proteins in rat dorsal root ganglia [J].
Fullmer, JM ;
Riedl, MS ;
Higgins, LA ;
Elde, R .
NEUROREPORT, 2004, 15 (11) :1705-1709