Altered Receptor Specificity and Cell Tropism of D222G Hemagglutinin Mutants Isolated from Fatal Cases of Pandemic A(H1N1) 2009 Influenza Virus

被引：163

作者：

Liu, Yan ^{[5
]}

Childs, Robert A. ^{[5
]}

Matrosovich, Tatyana ^{[1
]}

Wharton, Stephen ^{[2
]}

Palma, Angelina S. ^{[3
]}

Chai, Wengang ^{[5
]}

Daniels, Rodney ^{[2
]}

Gregory, Victoria ^{[2
]}

Uhlendorff, Jennifer ^{[1
]}

Kiso, Makoto ^{[4
]}

Klenk, Hans-Dieter ^{[1
]}

Hay, Alan

Feizi, Ten ^{[5
]}

Matrosovich, Mikhail ^{[1
]}

机构：

[1] Univ Marburg, Inst Virol, D-35043 Marburg, Germany

[2] Natl Inst Med Res, MRC, Div Virol, London NW7 1AA, England

[3] Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept Quim, REQUIMTE,Ctr Quim Fina & Biotecnol, P-2829516 Caparica, Portugal

[4] Gifu Univ, Dept Appl Bioorgan Chem, Fac Appl Biol Sci, Gifu 50111, Japan

[5] Univ London Imperial Coll Sci Technol & Med, Glycosci Lab, Dept Med, Harrow HA1 3UJ, Middx, England

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 22期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国惠康基金; 英国工程与自然科学研究理事会;

关键词：

AIRWAY EPITHELIAL-CELLS; MYCOPLASMA-PNEUMONIAE; A H1N1; BINDING PROPERTIES; SEVERE DISEASE; CARBOHYDRATE; SUBSTITUTION; MICROARRAY; INFECTION; MAMMALS;

D O I：

10.1128/JVI.01639-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutations in the receptor-binding site of the hemagglutinin of pandemic influenza A(H1N1) 2009 viruses have been detected sporadically. An Asp222Gly (D222G) substitution has been associated with severe or fatal disease. Here we show that 222G variants infected a higher proportion of ciliated cells in cultures of human airway epithelium than did viruses with 222D or 222E, which targeted mainly nonciliated cells. Carbohydrate microarray analyses showed that 222G variants bind a broader range of alpha 2-3-linked sialyl receptor sequences of a type expressed on ciliated bronchial epithelial cells and on epithelia within the lung. These features of 222G mutants may contribute to exacerbation of disease.

引用

页码：12069 / 12074

页数：6

共 24 条

[1]

[Anonymous], 2010, Wkly Epidemiol Rec, V85, P21

[2]

Bautista E, 2010, NEW ENGL J MED, V362, P1708, DOI 10.1056/NEJMra1000449

[3] Quasispecies of the D225G Substitution in the Hemagglutinin of Pandemic Influenza A(H1N1) 2009 Virus from Patients with Severe Disease in Hong Kong, China [J].

Chen, Honglin ;

Wen, Xi ;

To, Kelvin K. W. ;

Wang, Pui ;

Tse, Herman ;

Chan, Jasper F. W. ;

Tsoi, Hoi-Wah ;

Fung, Kitty S. C. ;

Tse, Cindy W. S. ;

Lee, Rodney A. ;

Chan, Kwok-Hung ;

Yuen, Kwok-Yung .

JOURNAL OF INFECTIOUS DISEASES, 2010, 201 (10) :1517-1521

[4] Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray [J].

Childs, Robert A. ;

Palma, Angelina S. ;

Wharton, Steve ;

Matrosovich, Tatyana ;

Liu, Yan ;

Chai, Wengang ;

Campanero-Rhodes, Maria A. ;

Zhang, Yibing ;

Eickmann, Markus ;

Kiso, Makoto ;

Hay, Alan ;

Matrosovich, Mikhail ;

Feizi, Ten .

NATURE BIOTECHNOLOGY, 2009, 27 (09) :797-799

[5] 6-sulfo sialyl Lewis X is the common receptor determinant recognized by H5, H6, H7 and H9 influenza viruses of terrestrial poultry [J].

Gambaryan, Alexandra S. ;

Tuzikov, Alexander B. ;

Pazynina, Galina V. ;

Desheva, Julia A. ;

Bovin, Nicolai V. ;

Matrosovich, Mikhail N. ;

Klimov, Alexander I. .

VIROLOGY JOURNAL, 2008, 5 (1)

[6] Effects of egg-adaptation on the receptor-binding properties of human influenza A and B viruses [J].

Gambaryan, AS ;

Robertson, JS ;

Matrosovich, MN .

VIROLOGY, 1999, 258 (02) :232-239

[7] H5N1 chicken influenza viruses display a high binding affinity for Neu5Acα2-3Galβ1-4(6-HSO3)GlcNAc-containing receptors [J].

Gambaryan, AS ;

Tuzikov, AB ;

Pazynina, GV ;

Webster, RG ;

Matrosovich, MN ;

Bovin, NV .

VIROLOGY, 2004, 326 (02) :310-316

[8] Influenza virus receptor specificity and cell tropism in mouse and human airway epithelial cells [J].

Ibricevic, Aida ;

Pekosz, Andrew ;

Walter, Michael J. ;

Newby, Celeste ;

Battaile, John T. ;

Brown, Earl G. ;

Holtzman, Michael J. ;

Brody, Steven L. .

JOURNAL OF VIROLOGY, 2006, 80 (15) :7469-7480

[9]

Kilander A, 2010, EUROSURVEILLANCE, V15, P6

[10] Mycoplasma pneumoniae host-pathogen studies in an air-liquid culture of differentiated human airway epithelial cells [J].

Krunkosky, Thomas M. ;

Jordan, Jarrat L. ;

Chambers, Emily ;

Krause, Duncan C. .

MICROBIAL PATHOGENESIS, 2007, 42 (2-3) :98-103

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