Classification of the mode of inhibition of high-affinity choline uptake using capillary electrophoresis with electrochemical detection at an enzyme-modified microelectrode

被引：7

作者：

Barkhimer, TV

Kirchhoff, JR

Hudson, RA

Messer, WS

机构：

[1] Univ Toledo, Coll Arts & Sci, Dept Chem, Toledo, OH 43606 USA

[2] Univ Toledo, Coll Pharm, Dept Med & Biol Chem, Toledo, OH 43606 USA

[3] Univ Toledo, Coll Pharm, Dept Pharmacol, Toledo, OH 43606 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 339卷 / 02期

关键词：

capillary electrophoresis; electrochemical detection; enzyme electrode; choline uptake assay; classification of inhibition;

D O I：

10.1016/j.ab.2005.01.047

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A nonradiochemical in vitro assay using capillary electrophoresis with electrochemical detection at an enzyme-modified microelectrode has been developed to evaluate the inhibition of high-affinity choline transport in synaptosomes. Quantitative analysis of high-affinity choline transporter rates Lis a function of inhibitor and substrate concentrations allowed determination of the mode of inhibition for the quaternary ammonium-catechol-based inhibitors 3-[(trimethylammonio)methyl]catechol, N,N-dimethylepinephrine, and 6-hydroxy-N,N-dimethlylepinephrine. The results are compared to the well-characterized inhibitor of choline transport, hemicholinium-3. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：216 / 222

页数：7

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