Physical ordering of six YACs from the RN region in pigs

被引:2
作者
Tornsten, A [1 ]
Jeon, JT [1 ]
Alexander, LJ [1 ]
Andersson, L [1 ]
Chowdhary, BP [1 ]
机构
[1] Swedish Univ Agr Sci, Dept Anim Breeding & Genet, S-75007 Uppsala, Sweden
关键词
fluorescent in situ hybridization; gene mapping; meat quality; pig; RN; YAC;
D O I
10.1046/j.1365-2052.1998.00340.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Six YAC clones representing five microsatellite markers from the RN region were mapped by fluorescent in situ hybridization (FISH) on pig metaphase chromosomes and their relative order was determined by pairwise multicolour FISH. Two of the microsatellites viz., Sw120 and Sw936 flank RN as well as the remaining three microsatellites Sw1683, Sw2083 and Sw1309. The results assigned the RN locus to the distal part of the 15q25 band. The linear order of the microsatellites was compared with the available linkage mapping data.
引用
收藏
页码:319 / 321
页数:3
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