Biofuel cell based on glucose oxidase and microperoxidase-11 monolayer-fundionalized electrodes

Apoglucose oxidase was reconstituted onto a pyrroloquinoline quinone and flavin:adenine dinucleotide phosphate, PQQ-FAD, monolayer associated with a rough Au electrode to yield a bioelectrocatalytically active glucose oxidase, GOx. Electrically contacted PQQ-FAD/GOx monolayer was applied to the biocatalytic oxidation of glucose. A microperoxidase-11, MP-11, monolayer was assembled onto a rough Au electrode and used for the biocatalytic reduction of H2O2. Both biocatalytic electrodes, Au/PQQ-FAD/GOx and Au/MP-11, were assembled into a biofuel cell using glucose and H2O2 as the fuel substrate and the oxidizer, respectively. The biofuel cell generates an open-circuit voltage,:voc, of a. 310 mV and a short-circuit current density, i(sc), of ca. 114 mu A cm(-2). The maximum electrical power, P-max, extracted from the cell is 32 mu W at an external optimal load of 3 k Omega. The fill factor of the biofuel cell, f= P-max I-sc(-1) V-oc(-1), is ca. 25%.