Low-temperature resistance of higher plants is significantly enhanced by a nonspecific cyanobacterial desaturase

被引：113

作者：

IshizakiNishizawa, O ^{[1
]}

Fujii, T ^{[1
]}

Azuma, M ^{[1
]}

Sekiguchi, K ^{[1
]}

Murata, N ^{[1
]}

Ohtani, T ^{[1
]}

Toguri, T ^{[1
]}

机构：

[1] NATL INST BASIC BIOL,OKAZAKI,AICHI 444,JAPAN

来源：

NATURE BIOTECHNOLOGY | 1996年 / 14卷 / 08期

关键词：

low-temperature resistance; transgenic plants; membrane lipids; Delta; 9; desaturase; cyanobacteria;

D O I：

10.1038/nbt0896-1003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A broad-specificity Delta 9 desaturase gene was cloned from the cyanobacterium Anacystis nidulans. The enzyme introduces a cis-double bond at the Delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduced into tobacco plants under transcriptional control of the cauliflower mosaic virus 35S promoter, and the enzyme was targeted into plastids by the transit peptide of the pea RuBisCO small subunit, The transgenic plants had a highly reduced level of saturated fatty acid content in most membrane lipids and exhibited a significant increase in chilling resistance.

引用

页码：1003 / 1006

页数：4

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BROWSE J, 1993, J BIOL CHEM, V268, P16345

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