3D morphometric analysis of calcified cartilage properties using micro-computed tomography

被引：21

作者：

Kauppinen, S. ^{[1
]}

Karhula, S. S. ^{[1
,2
]}

Thevenot, J. ^{[1
,2
]}

Ylitalo, T. ^{[3
]}

Rieppo, L. ^{[1
]}

Kestila, I. ^{[1
]}

Haapea, M. ^{[1
,9
]}

Hadjab, I. ^{[4
,5
]}

Finnila, M. A. ^{[1
,6
]}

Quenneville, E. ^{[5
]}

Garon, M. ^{[5
]}

Gahunia, H. K. ^{[7
]}

Pritzker, K. P. H. ^{[7
,8
]}

Buschmann, M. D. ^{[4
]}

Saarakkala, S. ^{[1
,9
]}

Nieminen, H. J. ^{[1
,3
,7
,10
]}

机构：

[1] Univ Oulu, Res Unit Med Imaging Phys & Technol, POB 5000, FI-90014 Oulu, Finland

[2] Univ Oulu, Infotech, Oulu, Finland

[3] Univ Helsinki, Dept Phys, Helsinki, Finland

[4] Polytech Montreal, Montreal, PQ, Canada

[5] Biomomentum Inc, 970 Michelin St,Suite 200, Laval, PQ H7L 5C1, Canada

[6] Univ Eastern Finland, Dept Appl Phys, Kuopio, Finland

[7] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[8] Mt Sinai Hosp, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[9] Oulu Univ Hosp, Dept Diagnost Radiol, Oulu, Finland

[10] Aalto Univ, Dept Neurosci & Biomed Engn, Espoo, Finland

来源：

OSTEOARTHRITIS AND CARTILAGE | 2019年 / 27卷 / 01期

基金：

芬兰科学院; 欧洲研究理事会;

关键词：

Osteoarthritis; Calcified cartilage; Tidemark; Morphology; Micro-computed tomography; Roughness; SUBCHONDRAL BONE; ARTICULAR-CARTILAGE; OSTEOARTHRITIS; MORPHOLOGY; PATHOGENESIS; CALCIFICATION; PROGRESSION; INITIATION; TIDEMARK; PLATE;

D O I：

10.1016/j.joca.2018.09.009

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Objective: Our aim is to establish methods for quantifying morphometric properties of calcified cartilage (CC) from micro-computed tomography (mu CT). Furthermore, we evaluated the feasibility of these methods in investigating relationships between osteoarthritis (OA), tidemark surface morphology and open subchondral channels (OSCCs). Method: Samples (n = 15) used in this study were harvested from human lateral tibial plateau (n = 8). Conventional roughness and parameters assessing local 3-dimensional (3D) surface variations were used to quantify the surface morphology of the CC. Subchondral channel properties (percentage, density, size) were also calculated. As a reference, histological sections were evaluated using Histopathological osteoarthritis grading (OARSI) and thickness of CC and subchondral bone (SCB) was quantified. Results: OARSI grade correlated with a decrease in local 3D variations of the tidemark surface (amount of different surface patterns (r(s) = -0.600, P = 0.018), entropy of patterns (EP) (r(s) = -0.648, P = 0.018), homogeneity index (HI) (r(s) = 0.555, P = 0.032)) and tidemark roughness (TMR) (r(s) = -0.579, P = 0.024). Amount of different patterns (ADP) and EP associated with channel area fraction (CAF) (r(p) = 0.876, P < 0.0001; r(p) = 0.665, P = 0.007, respectively) and channel density (CD) (r(p) = 0.680, P = 0.011; r(p) = 0.582, P = 0.023, respectively). TMR was associated with CAF (r(p) = 0.926, P < 0.0001) and average channel size (r(p) = 0.574, P = 0.025). CC topography differed statistically significantly in early OA vs healthy samples. Conclusion: We introduced a mu-CT image method to quantify 3D CC topography and perforations through CC. CC topography was associated with OARSI grade and OSCC properties; this suggests that the established methods can detect topographical changes in tidemark and CC perforations associated with OA. (c) 2018 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

引用

页码：172 / 180

页数：9

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