Cordycepin Induces Apoptosis of CGTH W-2 Thyroid Carcinoma Cells through the Calcium-Calpain-Caspase 7-PARP Pathway

被引：37

作者：

Chen, Ying ^{[1
]}

Chen, Yung-Chia ^{[1
]}

Lin, Yen-Tung ^{[1
]}

Huang, Shih-Horng ^{[2
]}

Wang, Seu-Mei ^{[1
]}

机构：

[1] Natl Taiwan Univ, Coll Med, Dept Anat & Cell Biol, Taipei 100, Taiwan

[2] Yuan Ze Univ, Grad Sch Biotechnol & Bioengn, Chungli, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 22期

关键词：

Cordycepin; thyroid carcinoma cells; apoptosis; signaling mechanism; ENDOPLASMIC-RETICULUM STRESS; A(1) ADENOSINE RECEPTORS; MOUSE MELANOMA-CELLS; CHO-CELLS; ACTIVATION; 3'-DEOXYADENOSINE; CANCER; INHIBITION; DEATH; SUPPRESSION;

D O I：

10.1021/jf1028976

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Cordycepin, a nucleoside isolated from Cordyceps sinensis, is an inhibitor of polyadenylation and has an antitumor effect. We used CGTH W-2, a follicular thyroid carcinoma cell line, to study the mechanism of the anticancer effect of cordycepin. Cordycepin decreased cell viability and resulted in apoptosis but not necrosis. Cordycepin increased intracellular calcium levels triggering calpain activation, which led to apoptosis. BAPTA/AM and calpeptin inhibited the cordycepin-induced cleavage of caspase 7 and poly (ADP-ribose) polymerase (PARP), implying an upstream role of calcium and calpain. CGTH W-2 cells expressed four subtypes of adenosine receptors (AR), A(1)AR, A(2A)AR, A(2B)AR, and A(3)AR. Specific antagonists to AR subtypes all blocked cordycepin-induced apoptosis to different degrees. Small interfering RNA for A(1)AR and A(3)AR abrogated cordycepin-induced apoptosis. In conclusion, the cordycepin-induced apoptosis of CGTH W-2 cells is mediated by the calcium-calpain-caspase 7-PARP pathway, and ARs are involved in the apoptotic effect of cordycepin.

引用

页码：11645 / 11652

页数：8

共 39 条

[1]

RNA-directed agent, cordycepin, induces cell death in multiple myeloma cells [J].

Chen, Lisa S. ;

Stellrecht, Christine M. ;

Gandhi, Varsha .

BRITISH JOURNAL OF HAEMATOLOGY, 2008, 140 (06) :682-691

[2]

Effects of PPARγ agonists on cell survival and focal adhesions in a Chinese thyroid carcinoma cell line [J].

Chen, Ying ;

Wang, Seu-Mei ;

Wu, Jiahn-Chun ;

Huang, Shih-Horng .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 98 (04) :1021-1035

[3]

Cordycepin (3′-deoxyadenosine) inhibits human platelet aggregation induced by U46619, a TXA2 analogue [J].

Cho, Hyun Jeong ;

Cho, Jae Youl ;

Rhee, Man Hee ;

Lim, Chang Ryul ;

Park, Hwa Jin .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2006, 58 (12) :1677-1682

[4]

Cross-talk between calpain and caspase-3/-7 in cisplatin-induced apoptosis of melanoma cells: a major role of calpain inhibition in cell death protection and p53 status [J].

Del Bello, B. ;

Moretti, D. ;

Gamberucci, A. ;

Maellaro, E. .

ONCOGENE, 2007, 26 (19) :2717-2726

[5]

Dunwiddie TV, 1997, J NEUROSCI, V17, P607

[6]

Long-term outcome of 444 patients with distant metastases from papillary and follicular thyroid carcinoma: Benefits and limits of radioiodine therapy [J].

Durante, C. ;

Haddy, N. ;

Baudin, E. ;

Leboulleux, S. ;

Hartl, D. ;

Travagli, J. P. ;

Caillou, B. ;

Ricard, M. ;

Lumbroso, J. D. ;

De Vathaire, F. ;

Schlumberger, M. .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2006, 91 (08) :2892-2899

[7]

Effector coupling of stably transfected human A3 adenosine receptors in CHO cells [J].

Englert, M ;

Quitterer, U ;

Klotz, KN .

BIOCHEMICAL PHARMACOLOGY, 2002, 64 (01) :61-65

[8]

FREUND S, 1994, N-S ARCH PHARMACOL, V350, P49

[9]

Calpain-1 Cleaves and Activates Caspase-7 [J].

Gafni, Juliette ;

Cong, Xin ;

Chen, Sylvia F. ;

Gibson, Bradford W. ;

Ellerby, Lisa M. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (37) :25441-25449

[10]

Cleavage of automodified poly(ADP-ribose) polymerase during apoptosis - Evidence for involvement of caspase-7 [J].

Germain, M ;

Affar, EB ;

D'Amours, D ;

Dixit, VM ;

Salvesen, GS ;

Poirier, GG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (40) :28379-28384

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