Recombination signatures distinguish embryonic stem cells derived by parthenogenesis and somatic cell nuclear transfer

被引：115

作者：

Kim, Kitai

Ng, Kitwa

Rugg-Gunn, Peter J.

Shieh, Jae-Hung

Kirak, Oktay

Jaenisch, Rudolf

Wakayama, Teruhiko

Moore, Malcolm A.

Pedersen, Roger A.

Daley, George Q.

机构：

[1] Childrens Hosp, Div Pediat Hematol, Boston, MA 02115 USA

[2] Dana Farber Canc Inst, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[4] Brigham & Womens Hosp, Div Hematol, Boston, MA 02115 USA

[5] Harvard Stem Cell Inst, Boston, MA 02115 USA

[6] Univ Cambridge, Dept Surg, Cambridge CB2 2XY, England

[7] Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 2XY, England

[8] Mem Sloan Kettering Canc Ctr, Cell Biol Program, New York, NY 10021 USA

[9] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[10] RIKEN Kobe, Ctr Dev Biol, Kobe, Hyogo 6500047, Japan

来源：

CELL STEM CELL | 2007年 / 1卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/j.stem.2007.07.001

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Parthenogenesis and somatic cell nuclear transfer (SCNT) are two methods for deriving embryonic stem (ES) cells that are genetically matched to the oocyte donor or somatic cell donor, respectively. Using genome-wide single nucleotide polymorphism (SNP) analysis, we demonstrate distinct signatures of genetic recombination that distinguish parthenogenetic ES cells from those generated by SCNT. We applied SNP analysis to the human ES cell line SCNT-hES-1, previously claimed to have been derived by SCNT, and present evidence that it represents a human parthenogenetic ES cell line. Genome-wide SNP analysis represents a means to validate the genetic provenance of an ES cell line.

引用

页码：346 / 352

页数：7

共 33 条

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