PKD is recruited to sites of actin remodelling at the leading edge and negatively regulates cell migration

被引：52

作者：

Eiseler, Tim ^{[1
]}

Schmid, Michael A. ^{[1
]}

Topbas, Fitnat ^{[1
]}

Pfizenmaier, Klaus ^{[1
]}

Hausser, Angelika ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Cell Biol & Immunol, D-70569 Stuttgart, Germany

来源：

FEBS LETTERS | 2007年 / 581卷 / 22期

关键词：

actin remodelling; cell migration; F-actin interaction; phosphorylation leading edge;

D O I：

10.1016/j.febslet.2007.07.079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein kinase D (PKD) has been implicated in the regulation of cell shape, adhesion, and migration. At the leading edge of migrating cells active PKD co-localizes with F-actin, Arp3 and cortactin. Platelet derived growth factor (PDGF) activates PKD and recruits the kinase to the leading edge, suggesting a role for PKD in actin remodelling. In support of this, PKD directly interacts with F-actin and phosphorylates cortactin in vitro. Interference with PKD function by overexpression of a dominant negative PKD or by PKD-specific siRNA enhanced cell migration, whereas cells overexpressing PKD wild type displayed reduced migratory potential. Taken together, these data reveal a negative regulatory function of PKD in cell migration. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4279 / 4287

页数：9

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