Tumor necrosis factor-α and -β upregulate the levels of osteoprotegerin mRNA in human osteosarcoma MG-63 cells

被引：87

作者：

Brändström, H

Jonsson, KB

Vidal, O

Ljunghall, S

Ohlsson, C

Ljunggren, Ö

机构：

[1] Univ Uppsala, Dept Med Sci, S-75185 Uppsala, Sweden

[2] Univ Goteborg, Dept Internal Med, S-41345 Gothenburg, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.8993

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Osteoprotegerin (OPG) is a recently cloned soluble member of the tumor necrosis factor receptor family. OPG has been shown to inhibit osteoclast recruitment by binding to OPG-ligand, an osteoclast differentiating factor on osteoblastic stromal cells, thereby blocking osteoclastogenesis. In this report we have examined the effect of tumor necrosis factor-alpha (TNF-alpha) and tumor necrosis factor-beta (TNF-beta) on OPG mRNA levels in the human osteosarcoma cell line MG-63. We demonstrate that both TNF-alpha and TNF-beta dose- and time-dependently upregulate the mRNA levels of OPG. The effect is significant at and above 5 pM of TNF-alpha and 1 pM of TNF-beta. The stimulatory effect on OPG mRNA levels in MG-63 cells was detected after 2 hrs of incubation with TNF-alpha or TNF-beta. These data demonstrate that the expression of OPC; in osteoblasts, with subsequent effects on osteoclastogenesis, is regulated by TNFs. (C) 1998 Academic Press.

引用

页码：454 / 457

页数：4

共 21 条

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