Circulating tumor cells: approaches to isolation and characterization

被引：871

作者：

Yu, Min ^{[1
,2
]}

Stott, Shannon ^{[3
]}

Toner, Mehmet ^{[3
]}

Maheswaran, Shyamala ^{[2
]}

Haber, Daniel A. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02129 USA

[2] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Canc, Boston, MA 02129 USA

[3] Harvard Univ, Sch Med, Ctr Engn Med, Boston, MA 02129 USA

来源：

JOURNAL OF CELL BIOLOGY | 2011年 / 192卷 / 03期

关键词：

BREAST-CANCER PATIENTS; RESISTANT PROSTATE-CANCER; EPITHELIAL-MESENCHYMAL TRANSITION; MULTIPARAMETER FLOW-CYTOMETRY; HUMAN PERIPHERAL-BLOOD; BONE-MARROW; LUNG-CANCER; FREE DNA; COLORECTAL-CANCER; CARCINOMA-CELLS;

D O I：

10.1083/jcb.201010021

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Circulating tumor cells (CTCs) shed from primary and metastatic cancers are admixed with blood components and are thus rare, making their isolation and characterization a major technological challenge. CTCs hold the key to understanding the biology of metastasis and provide a biomarker to noninvasively measure the evolution of tumor genotypes during treatment and disease progression. Improvements in technologies to yield purer CTC populations amenable to better cellular and molecular characterization will enable a broad range of clinical applications, including early detection of disease and the discovery of biomarkers to predict treatment responses and disease progression.

引用

页码：373 / 382

页数：10

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