Note on the analysis of ligand binding by the 'double-logarithmic' plot

被引:24
作者
Grossweiner, LI [1 ]
机构
[1] Advocate Ravenswood Hosp, Med Ctr, Wenske Laser Ctr, Chicago, IL 60640 USA
关键词
ligand binding;
D O I
10.1016/S1011-1344(00)00113-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The double-logarithmic plot has been employed for the fluorometric analysis of ligand binding. This analysis is valid only for 1:1 binding of a non-aggregating ligand and if the plot is based on the free, and not the total, ligand concentration. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:175 / 177
页数:3
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