Inactivation of Ebola virus and Middle East respiratory syndrome coronavirus in platelet concentrates and plasma by ultraviolet C light and methylene blue plus visible light, respectively

被引：102

作者：

Eickmann, Markus ^{[1
]}

Gravemann, Ute ^{[2
]}

Handke, Wiebke ^{[2
]}

Tolksdorf, Frank ^{[3
]}

Reichenberg, Stefan ^{[3
]}

Mueller, Thomas H. ^{[2
]}

Seltsam, Axel ^{[2
]}

机构：

[1] Philipps Univ Marburg, Inst Virol, Marburg, Germany

[2] German Red Cross Blood Serv NSTOB, Springe, Germany

[3] Macopharma Int GmbH, Langen, Germany

来源：

TRANSFUSION | 2018年 / 58卷 / 09期

关键词：

PATHOGEN REDUCTION; TRYPANOSOMA-CRUZI; BLOOD COMPONENTS; SYSTEM; EPIDEMIOLOGY; TECHNOLOGY; INFECTION; EFFICACY; UPDATE; SAFETY;

D O I：

10.1111/trf.14652

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

BACKGROUNDEbola virus (EBOV) and Middle East respiratory syndrome coronavirus (MERS-CoV) have been identified as potential threats to blood safety. This study investigated the efficacy of the THERAFLEX UV-Platelets and THERAFLEX MB-Plasma pathogen inactivation systems to inactivate EBOV and MERS-CoV in platelet concentrates (PCs) and plasma, respectively. STUDY DESIGN AND METHODSPCs and plasma were spiked with high titers of cell culture-derived EBOV and MERS-CoV, treated with various light doses of ultravioletC (UVC; THERAFLEX UV-Platelets) or methylene blue (MB) plus visible light (MB/light; THERAFLEX MB-Plasma), and assessed for residual viral infectivity. RESULTSUVC reduced EBOV (4.5 log) and MERS-CoV (3.7 log) infectivity in PCs to the limit of detection, and MB/light decreased EBOV (4.6 log) and MERS-CoV (3.3 log) titers in plasma to nondetectable levels. CONCLUSIONSBoth THERAFLEX UV-Platelets (UVC) and THERAFLEX MB-Plasma (MB/light) effectively reduce EBOV and MERS-CoV infectivity in platelets and plasma, respectively.

引用

页码：2202 / 2207

页数：6

共 32 条

[1]

[Anonymous], 2010, GES 2005 RICHTL

[2]

Treatment of blood with a pathogen reduction technology using ultraviolet light and riboflavin inactivates Ebola virus in vitro [J].

Cap, Andrew P. ;

Pidcoke, Heather F. ;

Keil, Shawn D. ;

Staples, Hilary M. ;

Anantpadma, Manu ;

Carrion, Ricardo, Jr. ;

Davey, Robert A. ;

Frazer-Abel, Ashley ;

Taylor, Audra L. ;

Gonzales, Richard ;

Patterson, Jean L. ;

Goodrich, Raymond P. .

TRANSFUSION, 2016, 56 :S6-S15

[3]

Castro E, 2008, VOX SANG, V95, P290

[4]

The efficacy of the ultraviolet C pathogen inactivation system in the reduction of Babesia divergens in pooled buffy coat platelets [J].

Castro, Emma ;

Miguel Gonzalez, Luis ;

Miguel Rubio, Jose ;

Ramiro, Raquel ;

Girones, Nuria ;

Montero, Estrella .

TRANSFUSION, 2014, 54 (09) :2207-2216

[5]

Viral Shedding and Antibody Response in 37 Patients With Middle East Respiratory Syndrome Coronavirus Infection [J].

Corman, Victor M. ;

Albarrak, Ali M. ;

Omrani, Ali Senosi ;

Albarrak, Mohammed M. ;

Farah, Mohamed Elamin ;

Almasri, Malak ;

Muth, Doreen ;

Sieberg, Andrea ;

Meyer, Benjamin ;

Assiri, Abdullah M. ;

Binger, Tabea ;

Steinhagen, Katja ;

Lattwein, Erik ;

Al-Tawfiq, Jaffar ;

Mueller, Marcel A. ;

Drosten, Christian ;

Memish, Ziad A. .

CLINICAL INFECTIOUS DISEASES, 2016, 62 (04) :477-483

[6]

Inter-strand photoproducts are produced in high yield within A-DNA exposed to UVC radiation [J].

Douki, T ;

Laporte, G ;

Cadet, J .

NUCLEIC ACIDS RESEARCH, 2003, 31 (12) :3134-3142

[7]

Identification of a novel coronavirus in patients with severe acute respiratory syndrome [J].

Drosten, C ;

Günther, S ;

Preiser, W ;

van der Werf, S ;

Brodt, HR ;

Becker, S ;

Rabenau, H ;

Panning, M ;

Kolesnikova, L ;

Fouchier, RAM ;

Berger, A ;

Burguière, AM ;

Cinatl, J ;

Eickmann, M ;

Escriou, N ;

Grywna, K ;

Kramme, S ;

Manuguerra, JC ;

Müller, S ;

Rickerts, V ;

Stürmer, M ;

Vieth, S ;

Klenk, HD ;

Osterhaus, ADME ;

Schmitz, H ;

Doerr, HW .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1967-1976

[8]

PLATELET CONCENTRATES IN AN ADDITIVE SOLUTION PREPARED FROM POOLED BUFFY COATS - INVIVO STUDIES [J].

ERIKSSON, L ;

SHANWELL, A ;

GULLIKSSON, H ;

HOGMAN, CF ;

SVENSSON, LA ;

KRISTENSEN, J ;

BERG, B .

VOX SANGUINIS, 1993, 64 (03) :133-138

[9]

Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light [J].

Faddy, Helen M. ;

Fryk, Jesse J. ;

Prow, Natalie A. ;

Watterson, Daniel ;

Young, Paul R. ;

Hall, Roy A. ;

Tolksdorf, Frank ;

Sumian, Chryslain ;

Gravemann, Ute ;

Seltsam, Axel ;

Marks, Denese C. .

TRANSFUSION, 2016, 56 (06) :1548-1555

[10]

Clinical recognition and management of patients exposed to biological warfare agents [J].

Franz, DR ;

Jahrling, PB ;

Friedlander, AM ;

McClain, DJ ;

Hoover, DL ;

Bryne, WR ;

Pavlin, JA ;

Christopher, CW ;

Eitzen, EM .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1997, 278 (05) :399-411

← 1 2 3 4 →