Production of acylated homoserine lactones by different serotypes of Vibrio anguillarum both in culture and during infection of rainbow trout

被引:37
作者
Buch, C
Sigh, J
Nielsen, J
Larsen, JL
Gram, L
机构
[1] Tech Univ Denmark, Danish Inst Fisheries Res, DK-2800 Lyngby, Denmark
[2] Royal Vet & Agr Univ, Dept Chem, DK-1871 Frederiksberg, Denmark
关键词
acylated homoserine lactones; infection studies; quorum sensing; rainbow trout; Vibrio anguillarum;
D O I
10.1078/072320203322497365
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Onehundred and forty-eight out of onehundred and fifty strains of Vibrio anguillarum isolated from vibriosis in Danish marine aquaculture produced bacterial communication signals, acylated homoserine lactones, eliciting a response in the Agrobacterium tumefaciens (pZLR4) monitoring system. One strain, a serotype O4, induced a strong response in the Chromobacterium violaceum (CV026) monitoring system. Profiles of AHLs determined by TLC separation revealed the presence of at least four AHLs and a compound similar to N-3-oxo-decanoyl homoserine lactone (3-oxo-C10-HSL) was present in all strains. The production rate of the presumed 3-oxo-C10-HSL followed the growth rate of V. anguillarum whereas the production rate of a small AHL (R-f value of 0.74) increased faster than the growth rate of V anguillarum indicating autoinduction. AHLs were produced by all serotypes (O1 to O10) and by non-typable strains. During infection with V anguillarum, AHLs could be extracted from liver, kidney and muscle of rainbow trout and AHLs were detected both in, vitro and in vivo when cell numbers reached 10(7) per ml or gram. Preliminary investigations of interactions between AHLs and the fish immune system were carried out determining oxidative burst of fish macrophages exposed to 3-oxo-C10-HSL. No activation or suppression of the superoxide anion production in the head kidney macrophages was seen when treated with the AHL compound in concentrations of 1 nM-10 muM. Our data show that AHLs are produced by almost all V. anguillarum strains and that no clear pattern relating AHL production to disease or virulence appear.
引用
收藏
页码:338 / 349
页数:12
相关论文
共 56 条
[1]   Study of a melanic pigment of Proteus mirabilis [J].
Agodi, A ;
Stefani, S ;
Corsaro, C ;
Campanile, F ;
Gribaldo, S ;
Sichel, G .
RESEARCH IN MICROBIOLOGY, 1996, 147 (03) :167-174
[2]  
[Anonymous], 1993, BACTERIAL FISH PATHO
[3]   LEUKOCYTE OXIDASE - DEFECTIVE ACTIVITY IN CHRONIC GRANULOMATOUS DISEASE [J].
BAEHNER, RL ;
NATHAN, DG .
SCIENCE, 1967, 155 (3764) :835-&
[4]   A GENERAL ROLE FOR THE LUX AUTOINDUCER IN BACTERIAL-CELL SIGNALING - CONTROL OF ANTIBIOTIC BIOSYNTHESIS IN ERWINIA [J].
BAINTON, NJ ;
BYCROFT, BW ;
CHHABRA, SR ;
STEAD, P ;
GLEDHILL, L ;
HILL, PJ ;
REES, CED ;
WINSON, MK ;
SALMOND, GPC ;
STEWART, GSAB ;
WILLIAMS, P .
GENE, 1992, 116 (01) :87-91
[5]   INTERCELLULAR SIGNALING IN VIBRIO-HARVEYI - SEQUENCE AND FUNCTION OF GENES REGULATING EXPRESSION OF LUMINESCENCE [J].
BASSLER, BL ;
WRIGHT, M ;
SHOWALTER, RE ;
SILVERMAN, MR .
MOLECULAR MICROBIOLOGY, 1993, 9 (04) :773-786
[7]   THE LUX AUTOINDUCER-RECEPTOR INTERACTION IN VIBRIO-HARVEYI - BINDING PARAMETERS AND STRUCTURAL REQUIREMENTS FOR THE AUTOINDUCER [J].
CAO, JG ;
WEI, ZY ;
MEIGHEN, EA .
BIOCHEMICAL JOURNAL, 1995, 312 :439-444
[8]  
CAO JG, 1989, J BIOL CHEM, V264, P21670
[9]   Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria [J].
Cha, C ;
Gao, P ;
Chen, YC ;
Shaw, PD ;
Farrand, SK .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (11) :1119-1129
[10]   Synthetic analogues of the bacterial signal (quorum sensing) molecule N-(3-oxododecanoyl)-L-homoserine lactone as immune modulators [J].
Chhabra, SR ;
Harty, C ;
Hooi, DSW ;
Daykin, M ;
Williams, P ;
Telford, G ;
Pritchard, DI ;
Bycroft, BW .
JOURNAL OF MEDICINAL CHEMISTRY, 2003, 46 (01) :97-104