Physical mapping of chromosome 6: A strategy for the rapid generation of sequence-ready contigs

被引：5

作者：

Mungall, AJ ^{[1
]}

Edwards, CA ^{[1
]}

Ranby, SA ^{[1
]}

Humphray, SJ ^{[1
]}

Heathcott, RW ^{[1
]}

Clee, CM ^{[1
]}

East, CL ^{[1
]}

Holloway, E ^{[1
]}

Butler, AP ^{[1
]}

Langford, CF ^{[1
]}

Gwilliam, R ^{[1
]}

Rice, KM ^{[1
]}

Maslen, GL ^{[1
]}

Carter, NP ^{[1
]}

Ross, MT ^{[1
]}

Deloukas, P ^{[1
]}

Bentley, DR ^{[1
]}

Dunham, I ^{[1
]}

机构：

[1] SANGER CTR,CAMBRIDGE CB10 ISA,ENGLAND

来源：

DNA SEQUENCE | 1996年 / 7卷 / 01期

关键词：

D O I：

10.3109/10425179609015647

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The development of radiation hybrid (RH) mapping (Cox et al., 1990) and the availability of large numbers of STS markers, together with extensive bacterial clone resources provided a means to accelerate the process of mapping a human chromosome and preparing bacterial clone contigs ready to sequence. Our aim is to construct physical clone maps covering those regions of chromosome 6 that are not currently extensively mapped, and use these to determine the DNA sequence of the whole chromosome. We report here a strategy which initially involves establishing a high density framework map using RH mapping. The framework markers are then used for the identification of bacterial genomic clones covering the chromosome. The bacterial clones are analysed by restriction enzyme fingerprinting and STS-content analysis to identify sequence-ready contigs. Contig gap closure will also be performed by clone walking.

引用

页码：47 / 49

页数：3

共 4 条

[1]

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[3] A radiation hybrid map of the human genome [J].

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Schmitt, K ;

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Spillett, D ;

Muselet, D ;

PrudHomme, JF ;

Dib, C ;

Auffray, C ;

Morissette, J ;

Weissenbach, J ;

Goodfellow, PN .

HUMAN MOLECULAR GENETICS, 1996, 5 (03) :339-346

[4]

IOANNOU PA, 1994, NAT GENET, V6, P84, DOI 10.1038/ng0194-84

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