The structure of a polysaccharide from infectious strains of Burkholderia cepacia

被引：123

作者：

Linker, A

Evans, LR

Impallomeni, G

机构：

[1] VA Salt Lake City Hlth Care Syst, Salt Lake City, UT 84124 USA

[2] Univ Utah, Dept Pathol, Salt Lake City, UT 84148 USA

[3] Univ Utah, Dept Biochem, Salt Lake City, UT 84148 USA

[4] CNR, Ist Chim & Tecnol Mat Polimer, I-95125 Catania, Italy

来源：

CARBOHYDRATE RESEARCH | 2001年 / 335卷 / 01期

关键词：

Burkholderia cepacia; opportunistic pathogen; enzymatic degradation; exopolysaccharide; structure; assignments; NMR;

D O I：

10.1016/S0008-6215(01)00205-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The structure of an acidic exopolysaccharide (EPS) from eight strains of Burkholderia cepacia has been investigated by methylation and sugar analysis, periodate oxidation-Smith degradation, and partial acid-hydrolysis. An enzyme preparation obtained from the same organisms producing the EPS was also used to depolymerize the polysaccharide. Detailed NMR studies of the chemical and enzymatic degradation products showed that this EPS consists of a highly branched heptasaccharide-repeating unit with the following structure: [GRAPHICS] About three O-acetyl groups per repeating unit are present at undetermined positions. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：45 / 54

页数：10

共 33 条

[1]

HOST-SYMBIONT INTERACTIONS .4. STRUCTURAL ELUCIDATION, USING HPLC-MS AND GLC-MS OF THE ACIDIC POLYSACCHARIDE SECRETED BY RHIZOBIUM-MELILOTI STRAIN-1021 [J].