Gene profiling and characterization of arginine kinase-1 (MrAK-1) from freshwater giant prawn (Macrobrachium rosenbergii)

被引：69

作者：

Arockiaraj, Jesu ^{[1
]}

Vanaraja, Puganeshwaran ^{[1
]}

Easwvaran, Sarasvathi ^{[1
]}

Singh, Arun ^{[2
]}

Alinejaid, Tahereh ^{[1
]}

Othman, Rofina Yasmin ^{[1
]}

Bhassu, Subha ^{[1
]}

机构：

[1] Univ Malaya, Ctr Biotechnol Agr Res, Div Genet & Mol Biol, Inst Biol Sci,Fac Sci, Kuala Lumpur 50603, Malaysia

[2] St Xaviers Coll Autonomous, Ctr Aquaculture Res & Extens, Palayankottai 627002, Tamil Nadu, India

来源：

FISH & SHELLFISH IMMUNOLOGY | 2011年 / 31卷 / 01期

关键词：

Arginine kinase; Macrobrachium rosenbergii; IHHNV virus; Gene expression; Kinetic activity; CUCUMBER STICHOPUS-JAPONICUS; AMINO-ACID-SEQUENCES; PHOSPHAGEN KINASE; FENNEROPENAEUS-CHINENSIS; LITOPENAEUS-VANNAMEI; CALLINECTES-SAPIDUS; BLUE-CRAB; EXPRESSION; SHRIMP; EVOLUTION;

D O I：

10.1016/j.fsi.2011.04.004

中图分类号：

S9 [水产、渔业];

学科分类号：

090805 [渔业资源学];

摘要：

Arginine kinase-1 (MrAK-1) was sequenced from the freshwater prawn Macrobrachium rosenbetgii using Illumina Solexa Genome Analyzer Technique. MrAK-1 consisted of 1068 bp nucleotide encoded 355 polypeptide with an estimated molecular mass of 40 kDa. MrAK-1 sequence contains a potential ATP:guanido phosphotransferases active domain site. The deduced amino acid sequence of MrAK-1 was compared with other 7 homologous arginine kinase (AK) and showed the highest identity (96%) with AK-1 from cherry shrimp Neocaridina denticulate. The qRT-PCR analysis revealed a broad expression of MrAK-1 with the highest expression in the muscle and the lowest in the eyestalk. The expression of MrAK-1 after challenge with the infectious hypodermal and hematopoietic necrosis virus (IHHNV) was tested in muscle. In addition, MrAK-1 was expressed in Escherichia coli by prokaryotic expression plasmid pMAL-c2x. The optimum temperature (30 degrees C) and pH (8.5) was determined for the enzyme activity assay. MrAK-1 showed significant (P < 0.05) activity towards 10-50 mM ATP concentration. The enzyme activity was inhibited by alpha-ketoglutarate, glucose and ATP at the concentration of 10, 50 and 100 mM respectively. Conclusively, the findings of this study indicated that MrAK-1 might play an important role in the coupling of energy production and utilization and the immune response in shrimps. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：81 / 89

页数：9

共 43 条

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