Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot

被引：145

作者：

Glienke, C. ^{[1
]}

Pereira, O. L. ^{[2
]}

Stringari, D. ^{[1
]}

Fabris, J. ^{[1
]}

Kava-Cordeiro, V. ^{[1
]}

Galli-Terasawa, L. ^{[1
]}

Cunnington, J. ^{[3
]}

Shivas, R. G. ^{[4
]}

Groenewald, J. Z. ^{[5
]}

Crous, P. W. ^{[5
,6
,7
]}

机构：

[1] Univ Fed Parana, Dept Genet, Ctr Politecn, BR-81531990 Curitiba, Parana, Brazil

[2] Univ Fed Vicosa, Dept Fitopatol, BR-36571000 Vicosa, MG, Brazil

[3] Dept Primary Ind Knoxfield, Melbourne, Vic 3156, Australia

[4] Ecosci Precinct, Agrisci Queensland, Dutton Pk, Qld 4102, Australia

[5] CBS KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands

[6] Univ Utrecht, Dept Biol, NL-3584 CH Utrecht, Netherlands

[7] Wageningen Univ & Res Ctr WUR, Lab Phytopathol, NL-6708 PB Wageningen, Netherlands

来源：

PERSOONIA | 2011年 / 26卷

关键词：

Guignardia endophyllicola; Guignardia mangiferae; Phyllosticta bifrenariae; Phyllosticta brazilianiae; Phyllosticta capitalensis; Phyllosticta citriasiana; Phyllosticta citribraziliensis; Phyllosticta citricarpa; taxonomy; FUNGUS GUIGNARDIA-CITRICARPA; PHYLOGENETIC LINEAGES; GENE GENEALOGIES; CALONECTRIA; PLANTS; MYCOSPHAERELLA; MANGIFERAE; DIVERSITY; ANAMORPHS; BANANA;

D O I：

10.3767/003158511X569169

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a taxon generally referred to as G. mangiferae, the presumed teleomorph of P. capitalensis, which is a non-pathogenic endophyte, commonly isolated from citrus leaves and fruits and a wide range of other hosts. DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S nrDNA, ITS2) and partial translation elongation factor 1-alpha (TEF1), actin and glyceraldehyde-3-phosphate dehydrogenase (GPDH) genes resolved nine clades correlating to seven known, and two apparently undescribed species. Phyllosticta citribraziliensis is newly described as an endophytic species occurring on Citrus in Brazil. An epitype is designated for P. citricarpa from material newly collected in Australia, which is distinct from P. citriasiana, presently only known on C. maxima from Asia. Phyllosticta bifrenariae is newly described for a species causing leaf and bulb spots on Bifrenaria harrisoniae (Orchidaceae) in Brazil. It is morphologically distinct from P. capitalensis, which was originally described from Stanhopea (Orchidaceae) in Brazil; an epitype is designated here. Guignardia mangiferae, which was originally described from Mangifera indica (Anacardiaceae) in India, is distinguished from the non-pathogenic endophyte, P. brazilianiae sp. nov., which is common on M. indica in Brazil. Furthermore, a combined phylogenetic tree revealed the P. capitalensis s.l. clade to be genetically distinct from the reference isolate of G. mangiferae. Several names are available for this clade, the oldest being P. capitalensis. These results suggest that endophytic, non-pathogenic isolates occurring on a wide host range would be more correctly referred to as P. capitalensis. However, more genes need to be analysed to fully resolve the morphological variation still observed within this clade.

引用

页码：47 / 56

页数：10

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