Rapid identification of novel genes expressed in a circadian manner in rat suprachiasmatic nuclei

被引：9

作者：

Chong, NWS ^{[1
]}

Cagampang, FRA ^{[1
]}

Coen, CW ^{[1
]}

Campbell, IC ^{[1
]}

Powell, JF ^{[1
]}

机构：

[1] UNIV LONDON KINGS COLL, DIV BIOMED SCI, ANAT & HUMAN BIOL GRP, STRAND, LONDON WC2R 2LS, ENGLAND

来源：

NEUROREPORT | 1996年 / 7卷 / 06期

关键词：

mRNA differential display; suprachiasmatic nucleus; rat; circadian rhythm; clock genes; gene expression; polymerase chain reaction; in situ hybridization;

D O I：

10.1097/00001756-199604260-00021

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE circadian clock in mammals is located in the suprachiasmatic nuclei (SCN). There is evidence that changes in gene expression are central to its mechanism. We are engaged in identifying the genes involved. The small size of the SCN, the large number of mammalian genes and the need to identify those differentially expressed over 24 h have required novel experimental procedures. mRNA differential display and an improved tissue micropunching method have been used to examine temporal changes in gene expression in the SCN of rats maintained in constant darkness. Several of the displayed cDNA species were found to be differentially expressed; they show no homology with published sequences. Riboprobes of these cDNA species were used for in situ hybridization. Emulsion-dipped sections confirmed that at least two of these differentially displayed mRNAs are expressed in a circadian manner.

引用

页码：1199 / 1203

页数：5

共 20 条

[1] TEMPORAL ORIENTATION - CIRCADIAN CLOCKS IN ANIMALS AND HUMANS [J].

ASCHOFF, J .

ANIMAL BEHAVIOUR, 1989, 37 :881-896

[2] DIFFERENTIAL EXPRESSION OF MESSENGER-RNA IN CHICK PINEAL OVER 24 HOURS [J].

CHONG, NWS ;

BADAKHCHANI, P ;

COEN, CW ;

CAMPBELL, IC ;

POWELL, JF .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1994, 22 (02) :S181-S181

[3]

DOEN CW, 1988, NEUROSCI LETT S, V32, pS15

[4]

DUNLAP JC, 1993, ANNU REV PHYSIOL, V55, P683, DOI 10.1146/annurev.ph.55.030193.003343

[5] IDENTIFICATION OF CIRCADIAN GENE-EXPRESSION IN THE RAT PINEAL-GLAND AND RETINA BY MESSENGER-RNA DIFFERENTIAL DISPLAY [J].

GAUER, F ;

KEDZIERSKI, W ;

CRAFT, CM .

NEUROSCIENCE LETTERS, 1995, 187 (01) :69-73

[6] METHODS FOR ASSESSING THE STATISTICAL SIGNIFICANCE OF MOLECULAR SEQUENCE FEATURES BY USING GENERAL SCORING SCHEMES [J].

KARLIN, S ;

ALTSCHUL, SF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (06) :2264-2268

[7]

KARSCH FJ, 1984, RECENT PROG HORM RES, V40, P185

[8] A SYSTEMATIC SURVEY OF REPETITIVE SEQUENCES ABUNDANTLY EXPRESSED IN RAT-TUMORS [J].

KOHNOE, S ;

MAEHARA, Y ;

ENDO, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 909 (02) :107-114

[9] DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION [J].

LIANG, P ;

PARDEE, AB .

SCIENCE, 1992, 257 (5072) :967-971

[10] DISTRIBUTION AND CLONING OF EUKARYOTIC MESSENGER-RNAS BY MEANS OF DIFFERENTIAL DISPLAY - REFINEMENTS AND OPTIMIZATION [J].

LIANG, P ;

AVERBOUKH, L ;

PARDEE, AB .

NUCLEIC ACIDS RESEARCH, 1993, 21 (14) :3269-3275

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