Immunophenotypic changes induced on human HL60 leukaemia cells by 1α,25-dihydroxyvitamin D3 and 12-O-tetradecanoyl phorbol-13-acetate

被引：42

作者：

White, SL

Belov, L

Barber, N

Hodgkin, PD

Christopherson, RI ^{[1
]}

机构：

[1] Univ Sydney, Sch Mol & Microbial Biosci, Sydney, NSW 2006, Australia

[2] Walter & Eliza Hall Inst Med Res, Melbourne, Vic 3050, Australia

来源：

LEUKEMIA RESEARCH | 2005年 / 29卷 / 10期

关键词：

myeloid leukaemia; vitamin D3; TPA; CID antigens; microarray;

D O I：

10.1016/j.leukres.2005.02.012

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

1 alpha,25-Dihydroxyvitamin D-3 (1,25D(3)) induces HL60 cells to acquire a monocyte-like phenotype, while cells treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA) resemble macrophages. Using a microarray of 82 CD antibodies, 24 cluster of differentiation (CD) antigens were detected on HL60 cells. 1,25D(3) induced the following antigens in decreasing order of the change: CD14, CD11c, CD11b, CD54, CD86, CD38 and CD66c, with repression of CD117, CD71, CD95, CD45 and CD64. TPA induced the following antigens in decreasing order of the change: CD11c, CD9, CD11b, CD54, CD38, CD45RO and CD66c, with repression of CD4, CD117, CD95, CD71 and CD64. The results presented provide a basis for monitoring differentiation therapy of myeloid leukaemias in patients. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1141 / 1151

页数：11

共 62 条

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