Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4

被引：6

作者：

Hosaka, T ^{[1
]}

Ui, S ^{[1
]}

Mimura, A ^{[1
]}

机构：

[1] Univ Yamanashi, Fac Engn, Dept Appl Chem & Biotechnol, Yamanashi 4008511, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 01期

关键词：

acetylacetoin; acetylacetoin reductase; acetylbutanediol; 2,3-butanediol cycle;

D O I：

10.1271/bbb.63.199

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation and purification of two kinds of acetylacetoin reductases (AACRs) from Bacillus cereus YUF-4 were examined, NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium sulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chromatography, The former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.

引用

页码：199 / 201

页数：3

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