Asymmetric responsiveness to ethylene mediates cell elongation in the apical hook of peas

被引:42
作者
Peck, SC
Pawlowski, K
Kende, H [1 ]
机构
[1] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA
[2] Wageningen Agr Univ, Dept Mol Biol, NL-6703 HA Wageningen, Netherlands
关键词
D O I
10.1105/tpc.10.5.713
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The apical hook of dark-grown dicotyledonous seedlings is a protective structure resulting from an inhibition of cell elongation on the inner portion of the hook. This differential growth response is mediated by ethylene, Expression of the gene encoding 1-aminocyclopropane-1-carboxylate oxidase (ACO), the terminal enzyme in ethylene biosynthesis, is induced by ethylene via a positive feedback loop. Therefore, the ACO transcript can serve as a molecular marker for both ethylene formation and ethylene responsiveness. We examined the distribution of ACO mRNA of pea, Ps-ACO1, and of ACO enzyme activity in the apical hook of etiolated pea seedlings. In situ hybridization showed that cells on the inner, concave side of pea hooks accumulated more Ps-ACO1 mRNA than did cells on the outer, convex side. The distribution of ACO enzyme activity followed the same pattern. A direct correlation was observed between the cellular distribution of Ps-ACO1 mRNA, ACO enzyme activity, and the inhibition of cell elongation. Pea seedlings treated with a saturating concentration of ethylene still accumulated higher levels of the Ps-ACO1 transcript on the inner side of the apical hook, demonstrating an increased responsiveness to ethylene in this tissue. These results indicate that an asymmetrically distributed component of the ethylene signal transduction pathway mediates hook formation. Based on existing genetic evidence, we propose that this component is downstream from the serine/threonine protein kinase CTR1.
引用
收藏
页码:713 / 719
页数:7
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