EbpR is important for biofilm formation by activating expression of the endocarditis and biofilm-associated pilus operon (ebpABC) of Enterococcus faecalis OG1RF

被引：45

作者：

Bourgogne, Agathe

Singh, Kavindra V.

Fox, Kristina A.

Pflughoeft, Kathryn J.

Murray, Barbara E.

Garsin, Danielle A.

机构：

[1] Univ Texas, Sch Med, Div Infect Dis, Ctr Study Emerging & Reemerging Pathogens, Houston, TX 77030 USA

[2] Univ Texas, Sch Med, Div Infect Dis, Dept Med, Houston, TX 77030 USA

[3] Univ Texas, Sch Med, Dept Microbiol & Mol Genet, Houston, TX 77030 USA

来源：

JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 17期

关键词：

GRAM-POSITIVE BACTERIA; SERINE-PROTEASE; CONSTRUCTION; GELATINASE; SYSTEM; GENES;

D O I：

10.1128/JB.00594-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We identify ef1090 (renamed ebpR) and show its importance for the transcriptional regulation of expression of the Enterococcus faecalis pilus operon, ebpABC. An ebpR deletion (Delta ebpR) mutant was found to have reduced ebpABC expression with loss of pilus production and a defect in primary adherence with, as a consequence, reduced biofilm formation.

引用

页码：6490 / 6493

页数：4

共 17 条

[1] Transcriptional response of Enterococcus faecalis V583 to erythromycin [J].

Aakra, Å ;

Vebo, H ;

Snipen, L ;

Hirt, H ;

Aastveit, A ;

Kapur, V ;

Dunny, G ;

Murray, B ;

Nes, IF .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (06) :2246-2259

[2]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[3] Comparison of OG1RF and an isogenic fsrB deletion mutant by transcriptional analysis:: the Fsr system of Enterococcus faecalis is more than the activator of gelatinase and serine protease [J].

Bourgogne, A ;

Hilsenbeck, SG ;

Dunny, GM ;

Murray, BE .

JOURNAL OF BACTERIOLOGY, 2006, 188 (08) :2875-2884

[4] Improved vectors for nisin-controlled expression in gram-positive bacteria [J].

Bryan, EM ;

Bae, T ;

Kleerebezem, H ;

Dunny, GM .

PLASMID, 2000, 44 (02) :183-190

[5] atxA controls Bacillus anthracis capsule synthesis via acpA and a newly discovered regulator, acpB [J].

Drysdale, M ;

Bourgogne, A ;

Hilsenbeck, SG ;

Koehler, TM .

JOURNAL OF BACTERIOLOGY, 2004, 186 (02) :307-315

[6] Construction of an Enterococcus faecalis Tn917-mediated-gene-disruption library offers insight into Tn917 insertion patterns [J].

Garsin, DA ;

Urbach, J ;

Huguet-Tapia, JC ;

Peters, JE ;

Ausubel, FM .

JOURNAL OF BACTERIOLOGY, 2004, 186 (21) :7280-7289

[7] Molecular analysis of the Enterococcus faecalis serotype 2 polysaccharide determinant [J].

Hancock, LE ;

Shepard, BD ;

Gilmore, MS .

JOURNAL OF BACTERIOLOGY, 2003, 185 (15) :4393-4401

[8] Development of a host-genotype-independent counterselectable marker and a high-frequency conjugative delivery system and their use in genetic analysis of Enterococcus faecalis [J].

Kristich, Christopher J. ;

Chandler, Josephine R. ;

Dunny, Gary M. .

PLASMID, 2007, 57 (02) :131-144

[9] A general system for generating unlabelled gene replacements in bacterial chromosomes [J].

Leenhouts, K ;

Buist, G ;

Bolhuis, A ;

tenBerge, A ;

Kiel, J ;

Mierau, I ;

Dabrowska, M ;

Venema, G ;

Kok, J .

MOLECULAR & GENERAL GENETICS, 1996, 253 (1-2) :217-224

[10]

Malani PN, 2002, ENTEROCOCCI: PATHOGENESIS, MOLECULAR BIOLOGY, AND ANTIBIOTIC RESISTANCE, P385

← 1 2 →