Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris

被引：98

作者：

Lee, Jong Seok ^{[1
]}

Kwon, Jeong Seok ^{[1
]}

Won, Dong Pil ^{[1
]}

Lee, Keun Eok ^{[1
]}

Shin, Won Cheol ^{[1
]}

Hong, Eock Kee ^{[1
]}

机构：

[1] Kangwon Natl Univ, Coll Engn, Dept Bioengn & Technol, Chunchon 200701, Gangwon Do, South Korea

来源：

CARBOHYDRATE POLYMERS | 2010年 / 82卷 / 03期

关键词：

Cordyceps militaris; Immunostimulating polysaccharide; Macrophage activation; Random coil conformation; ANTITUMOR-ACTIVITY; HERICIUM-ERINACEUS; FRUITING BODIES; BETA-GLUCANS; SINENSIS; (1->3)-BETA-D-GLUCAN; ELUCIDATION; MECHANISMS; INDUCTION; POLYMERS;

D O I：

10.1016/j.carbpol.2010.06.025

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The water-soluble crude polysaccharides obtained from the liquid culture broth of Cordyceps militaris by ethanol precipitation were fractionated by DEAE cellulose and Sepharose CL-6B column chromatography. This fractionation process resulted in three polysaccharide fractions that were termed CPSN Fr I, CPSN Fr II, and CPSN Fr III. Of the fractions, CPSN Fr II was able to upregulate the functional events mediated by activated macrophages, such as production of nitric oxide (NO) and expression of cytokines (IL-1 beta and TNF-alpha). Its structural characteristics were investigated by a combination of chemical and instrumental analyses, including methylation, reductive cleavage, acetylation, Fourier transform infrared spectroscopy (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Results indicate that CPSN Fr II was a 1,6-branched-glucogalactomannan that had a molecular weight of 36 kDa. The configuration of the beta-linkage and random coil conformation of CPSN Fr II were confirmed using a Fungi-Fluor kit and congo red reagent, respectively. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：982 / 988

页数：7

共 38 条

[1]

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