Telomere length status of somatic cell sheep clones and their offspring

被引：19

作者：

Alexander, Basil

Coppola, Gianfranco

Perrault, Steven D.

Peura, Teija T.

Betts, Dean H.

King, W. Allan ^{[1
]}

机构：

[1] Univ Guelph, Ontario Vet Coll, Dept Biomed Sci, Guelph, ON N1G 2W1, Canada

[2] Turretfield Res Ctr, S Australian Res & Dev Inst, Rosedale, SA, Australia

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 2007年 / 74卷 / 12期

关键词：

telomere; somatic cell nuclear transfer; chromosomal abnormalities; LIFE-SPAN; NUCLEAR TRANSFER; IN-VITRO; RECONSTITUTION; CONSERVATION; ASSOCIATION; ELONGATION; EXTENSION; OVERHANG;

D O I：

10.1002/mrd.20735

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was carried out to determine the telomere length status of sheep clones and their offspring, and to examine telomere dynamics and chromosomal abnormalities in culture propagated donor cells. Skin samples were collected from somatic cell nuclear transfer-derived sheep clones, and three of their progeny generated by natural mating. Samples were collected from control animals (n = 35), spanning in age from 1 month to 36 months of age. Genomic DNA was extracted from cell/tissue samples and their telomere lengths were assessed by terminal restriction fragment (TRF) analysis. Results revealed: that (a) sheep clones derived from cultured somatic cells have shortened telomere lengths compared to age-matched controls; (b) the offspring derived from natural mating between clones had normal telomere lengths compared to their age-matched counterparts; and donor cell cultures beyond 20 population doublings had significantly (P<0.05) shortened telomeres and exhibited a higher numerical and structural chromosomal abnormalities.

引用

页码：1525 / 1537

页数：13

共 52 条

[31] Evidence that exposure of the telomere 3′ overhang sequence induces senescence [J].