The Drosophila melanogaster sperm proteome-II (DmSP-II)

被引：121

作者：

Wasbrough, Elizabeth R. ^{[2
]}

Dorus, Steve ^{[2
]}

Hester, Svenja ^{[3
]}

Howard-Murkin, Julie ^{[3
]}

Lilley, Kathryn ^{[3
]}

Wilkin, Elaine ^{[2
]}

Polpitiya, Ashoka ^{[4
]}

Petritis, Konstantinos ^{[4
]}

Karr, Timothy L. ^{[1
]}

机构：

[1] Arizona State Univ, Biodesign Inst, Ctr Infect Dis & Vaccinol & Evolutionary Med & In, Tempe, AZ 85287 USA

[2] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England

[3] Univ Cambridge, Cambridge Ctr Prote, Cambridge, England

[4] Translat Genom Res Inst, Ctr Prote, Phoenix, AZ 85004 USA

来源：

JOURNAL OF PROTEOMICS | 2010年 / 73卷 / 11期

基金：

美国国家卫生研究院;

关键词：

Shotgun proteomics; Genomics; Spermatogenesis; Evolution; Fertility; TANDEM MASS-SPECTROMETRY; IPG STRIP PREFRACTIONATION; LC-MS/MS IDENTIFICATION; Y-CHROMOSOME; MEMBRANE-PROTEIN; STATISTICAL-MODEL; FERTILITY FACTORS; GENES; DATABASE; REVEALS;

D O I：

10.1016/j.jprot.2010.09.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Advances in mass spectrometry technology, high-throughput proteomics and genome annotations have resulted in significant increases in our molecular understanding of sperm composition. Using improved separation and detection methods and an updated genome annotation, a re-analysis of the Drosophila melanogaster sperm proteome (DmSP) has resulted in the identification of 956 sperm proteins. Comparative analysis with our previous proteomic dataset revealed 766 new proteins and an updated sperm proteome containing a total of 1108 proteins, termed the DmSP-II. This expanded dataset includes additional proteins with predicted sperm functions and confirms previous findings concerning the genomic organization of sperm loci. Bioinformatic and protein network analyses demonstrated high quality and reproducibility of proteome coverage across three replicate mass spectrometry runs. The use of whole-cell proteomics in conjunction with characterized phenotypes, functional annotations and pathway information has advanced our systems level understanding of sperm proteome functional networks. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：2171 / 2185

页数：15

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