Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer

被引：257

作者：

Kondo, Jumpei ^{[1
,4
]}

Endo, Hiroko ^{[1
]}

Okuyama, Hiroaki ^{[1
]}

Ishikawa, Osamu ^{[2
]}

Iishi, Hiroyasu ^{[3
]}

Tsujii, Masahiko ^{[4
]}

Ohue, Masayuki ^{[2
]}

Inoue, Masahiro ^{[1
]}

机构：

[1] Osaka Med Ctr Canc & Cardiovasc Dis, Dept Biochem, Higashinari Ku, Osaka 5378511, Japan

[2] Osaka Med Ctr Canc & Cardiovasc Dis, Dept Surg, Higashinari Ku, Osaka 5378511, Japan

[3] Osaka Med Ctr Canc & Cardiovasc Dis, Dept Gastroenteral Oncol, Higashinari Ku, Osaka 5378511, Japan

[4] Osaka Univ, Grad Sch Med, Dept Gastroenterol & Hepatol, Suita, Osaka 5650871, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 15期

关键词：

colon; drug sensitivity; GROWTH-FACTOR RECEPTOR; CARCINOMA-CELLS; TUMOR-CELLS; DRUG SCREEN; SURVIVAL; ACTIVATION; EXPRESSION; ADHESION; GENE; APOPTOSIS;

D O I：

10.1073/pnas.1015938108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Primary culture of the cancer cells from patients' tumors can provide crucial information of individual tumors, yet the technology has not been optimized until now. We developed an innovative culture method for primary colorectal cancer cells, based on the principle that cell-cell contact of cancer cells was maintained throughout the process. When tumor tissue was dissociated into cell clusters, in which cell-cell contact was retained, they rapidly formed spheroids that we termed cancer tissue-originated spheroids (CTOSs). CTOSs of colorectal cancer consisted of highly purified and viable cancer cells, and they were prepared with high efficiency. In immunodeficient mice, CTOSs formed xenograft tumors that retained the features of the parental tumors. Moreover, CTOSs were able to be cultured and expanded in vitro using a 3D culture system and stem cell culture medium. This method allowed evaluation of chemosensitivity and signal pathway activation in cancer cells from individual patients. Easy preparation and culture of pure primary cancer cells provides an innovative platform for studying cancer biology and developing personalized medicine.

引用

页码：6235 / 6240

页数：6

共 35 条

[1] American Society of Clinical Oncology Provisional Clinical Opinion: Testing for KRAS Gene Mutations in Patients With Metastatic Colorectal Carcinoma to Predict Response to Anti-Epidermal Growth Factor Receptor Monoclonal Antibody Therapy [J].

Allegra, Carmen J. ;

Jessup, J. Milburn ;

Somerfield, Mark R. ;

Hamilton, Stanley R. ;

Hammond, Elizabeth H. ;

Hayes, Daniel F. ;

McAllister, Pamela K. ;

Morton, Roscoe F. ;

Schilsky, Richard L. .

JOURNAL OF CLINICAL ONCOLOGY, 2009, 27 (12) :2091-2096

[2] It's Diagnostics, Stupid [J].

Bernards, Rene .

CELL, 2010, 141 (01) :13-17

[3] Gene expression perturbation in vitro - A growing case for three-dimensional (3D) culture systems [J].

Birgersdotter, A ;

Sandberg, R ;

Ernberg, I .

SEMINARS IN CANCER BIOLOGY, 2005, 15 (05) :405-412

[4] Breast cancer cell lines: friend or foe? [J].

Burdall, SE ;

Hanby, AM ;

Lansdown, MRJ ;

Speirs, V .

BREAST CANCER RESEARCH, 2003, 5 (02) :89-95

[5] Anoikis: A necessary death program for anchorage-dependent cells [J].

Chiarugi, Paola ;

Giannoni, Elisa .

BIOCHEMICAL PHARMACOLOGY, 2008, 76 (11) :1352-1364

[6] E-cadherin directly contributes to PI3K/AKT activation by engaging the PI3K-p85 regulatory subunit to adherens junctions of ovarian carcinoma cells [J].

De Santis, G. ;

Miotti, S. ;

Mazzi, M. ;

Canevari, S. ;

Tomassetti, A. .

ONCOGENE, 2009, 28 (09) :1206-1217

[7] The MAPK signalling pathways and colorectal cancer [J].

Fang, JY ;

Richardson, BC .

LANCET ONCOLOGY, 2005, 6 (05) :322-327

[8] Spheroid-based drug screen: considerations and practical approach [J].

Friedrich, Juergen ;

Seidel, Claudia ;

Ebner, Reinhard ;

Kunz-Schughart, Leoni A. .

NATURE PROTOCOLS, 2009, 4 (03) :309-324

[9] DISRUPTION OF EPITHELIAL CELL-MATRIX INTERACTIONS INDUCES APOPTOSIS [J].

FRISCH, SM ;

FRANCIS, H .

JOURNAL OF CELL BIOLOGY, 1994, 124 (04) :619-626

[10]

GUSTERSON BA, 1982, CANCER RES, V42, P4763

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