Comparisons of microsatellite variability and population genetic structure of two endangered wild rice species, Oryza rufipogon and O-officinalis, and their conservation implications

被引:17
作者
Gao, LZ [1 ]
Zhang, CH [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Crop Germplasm Resources, Minist Agr, Key Lab Crop Germplasm & Biotechnol, Beijing, Peoples R China
基金
中国国家自然科学基金; 美国国家科学基金会;
关键词
conservation management; Oryza officinalis; Oryza rufipogon; population genetic structure; rice microsatellites;
D O I
10.1007/s10531-004-0537-y
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Conserving endangered wild rice species requires a thorough understanding of their population genetic structure and appropriate approaches. We applied six and seven microsatellite loci to study the genetic structure of six populations throughout the range of Chinese Oryza rufipogon and Oryza officinalis, respectively. The results showed that O. rufipogon possesses higher levels of genetic diversity but lower differentiation ( R-S = 3.2713, P = 100.0%, H-O = 0.1401, H-S = 0.5800, F-ST = 0.271) than O. officinalis ( R-S = 2.0545, P = 57.14%, H-O = 0.0470, H-S = 0.2830, F-ST = 0.554). Mean population F-IS was slightly larger for O. officinalis ( F-IS = 0.844) than that for O. rufipogon ( F-IS = 0.755), indicating that O. officinalis has slightly higher departures from Hardy - Weinberg expectations and heterozygosity deficits than O. rufipogon. In addition to different origins and evolutionary histories, O. officinalis has restricted gene. flow, high inbreeding, isolated small populations and fewer opportunities of hybridization with other taxa, which may determine major differences in population genetic structure from O. rufipogon. Our results suggest the adoption of a plan of involving fewer populations but more individuals within populations for O. rufipogon, while both the number of populations and the individuals for a sampled population should be almost equally considered for O. officinalis. The known high degree of inbreeding in the populations of both species implies that conservation and restoration genetics should particularly focus on the maintenance of historically significant processes such as high levels of outbreeding, gene. flow and large effective population sizes. We. finally proposed to further estimate the role of rice gene. flow in the conservation of O. rufipogon, and to perform detailed analysis of mating systems in both species for better conservation perspectives of their ecological and evolutionary processes.
引用
收藏
页码:1663 / 1679
页数:17
相关论文
共 77 条
  • [1] Microsatellite DNA markers for rice chromosomes
    Akagi, H
    Yokozeki, Y
    Inagaki, A
    Fujimura, T
    [J]. THEORETICAL AND APPLIED GENETICS, 1996, 93 (07) : 1071 - 1077
  • [2] Population genetic structure of wild rice Oryza glumaepatula distributed in the Amazon flood area influenced by its life-history traits
    Akimoto, M
    Shimamoto, Y
    Morishima, H
    [J]. MOLECULAR ECOLOGY, 1998, 7 (10) : 1371 - 1381
  • [3] BARBIER P, 1989, JAPAN J GENETICS, V7, P259
  • [4] BARBIER P, 1989, JAPAN J GENETICS, V7, P273
  • [5] BARRETT SCH, 1991, GENETICS AND CONSERVATION OF RARE PLANTS, P3
  • [6] Analysis of genetic variability of South American wild rice populations (Oryza glumaepatula) with isozymes and RAPD markers
    Buso, GSC
    Rangel, PH
    Ferreira, ME
    [J]. MOLECULAR ECOLOGY, 1998, 7 (01) : 107 - 117
  • [7] High genetic differentiation among remnant populations of the endangered Caesalpinia echinata Lam. (Leguminosae-Caesalpinioideae)
    Cardoso, MA
    Provan, J
    Powell, W
    Ferreira, PCG
    De Oliveira, DE
    [J]. MOLECULAR ECOLOGY, 1998, 7 (05) : 601 - 608
  • [8] DETECTION OF GENETIC-VARIATION BETWEEN AND WITHIN POPULATIONS OF GLIRICIDIA-SEPIUM AND G-MACULATA USING RAPD MARKERS
    CHALMERS, KJ
    WAUGH, R
    SPRENT, JI
    SIMONS, AJ
    POWELL, W
    [J]. HEREDITY, 1992, 69 : 465 - 472
  • [9] CONSERVATION OF RICE GENETIC-RESOURCES - LUXURY OR NECESSITY
    CHANG, TT
    [J]. SCIENCE, 1984, 224 (4646) : 251 - 256
  • [10] Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.)
    Chen, X
    Temnykh, S
    Xu, Y
    Cho, YG
    McCouch, SR
    [J]. THEORETICAL AND APPLIED GENETICS, 1997, 95 (04) : 553 - 567